Organic Transistors Incorporating Lipid Monolayers for Drug Interaction Studies
Organic Transistors Incorporating Lipid Monolayers for Drug Interaction Studies
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DOI：
10.1002/admt.201900680
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发表时间：
2019-10-18
影响因子：
6.8
通讯作者：
Owens, Roisin M.
中科院分区：
文献类型：
--
作者：
Cavassin, Priscila;Pappa, Anna-Maria;Owens, Roisin M.
细胞膜是细胞功能的基础，因为它们保护细胞并控制进出细胞的通道。尽管细胞膜具有明确的意义，但由于它们的复杂性和缺乏可用的技术来与它们接触并传导它们的功能，细胞膜通常很难研究。通过开发简单的简化模型来克服这种复杂性可以促进他们的研究。事实上，脂质层代表了一个简化但具有代表性的细胞膜模型。脂质层具有高度绝缘性，这一特性直接受到脂质填充或膜流动性变化的影响。膜模型中的这种物理变化可以通过与电子换能器耦合来表征。本发明将稳定在两种不混溶溶剂之间的脂质单层与有机电化学晶体管集成在一起，该晶体管能够在两相溶剂混合物中工作。该平台用于评估利多卡因（一种广泛使用的麻醉剂，其工作机制仍有争议）如何与细胞膜相互作用。本研究提供的证据表明，麻醉剂直接与膜中的脂质相互作用，影响其堆积，从而影响单层通透性。所提出的平台为研究复合膜相互作用提供了一个优雅的解决方案。
Cell membranes are fundamental for cellular function as they protect the cell and control passage in and out of the cell. Despite their clear significance, cell membranes are often difficult to study, due to their complexity and the lack of available technologies to interface with them and transduce their functions. Overcoming this complexity by developing simple, reductionist models can facilitate their study. Indeed, lipid layers represent a simplified yet representative model for a cell membrane. Lipid layers are highly insulating, a property that is directly affected by changes in lipid packing or membrane fluidity. Such physical changes in the membrane models can be characterized by coupling them with an electronic transducer. Herein, a lipid monolayer that is stabilized between two immiscible solvents is integrated with an organic electrochemical transistor, which is capable of operating in a biphasic solvent mixture. The platform is used to evaluate how lidocaine, a widely used anesthetic the working mechanism of which is still a matter of debate, interacts with the cell membrane. The present study provides evidence that the anesthetic directly interacts with the lipids in the membrane, affecting their packing and therefore the monolayer permeability. The proposed platform provides an elegant solution for studying compound-membrane interactions.