Human chronic myeloid leukemic cell line with positive Philadelphia chromosome exhibits megakaryocytic and erythroid characteristics.
Human chronic myeloid leukemic cell line with positive Philadelphia chromosome exhibits megakaryocytic and erythroid characteristics.
复制标题
费城染色体阳性的人慢性粒细胞白血病细胞系表现出巨核细胞和红细胞特征。
作者：
D. Seigneurin;P. Champelovier;G. Mouchiroud;R. Berthier;D. Leroux;M. Prenant;J. McGregor;J. Starck;F. Morlé;C. Micouin
从急性期慢性髓性白血病患者的血液中建立了一个细胞系（LAMA-84）。LAMA-84细胞保留了患者的染色体异常，即除18号染色体外的所有染色体都有三倍复制，4-5份存在费城（Ph）染色体，并且存在染色体标记。LAMA-84细胞具有未分化胚细胞的形态特征，但分析表明其属于巨核细胞谱系；8.5%的细胞检测到血小板过氧化物酶（PPO）；LAMA-84细胞与血小板糖蛋白（GP） IIb， IIIa和GPIIb/IIIa复合物的多克隆和单克隆抗体自发反应，交叉免疫电泳证实了其存在。LAMA-84细胞缺乏淋巴样细胞和成熟粒细胞的膜特征，但确实与某些针对未成熟髓样细胞的抗体反应。此外，它们的抗糖蛋白抗体呈阳性，并含有α -和γ -珠蛋白mRNA，因此显示出红系标志物的表达。因此，LAMA-84是一种三能、巨核细胞、红细胞和粒细胞细胞系。添加DMSO、丁酸盐、TPA和血红素后，巨核细胞和红细胞标记增强。LAMA-84细胞系是研究巨核细胞和红细胞分化以及肿瘤生长机制的一个有趣的工具。
A cell line (LAMA-84) has been established from the blood of a patient with chronic myeloid leukemia in acute phase. LAMA-84 cells retained the patient's chromosome abnormalities, i.e., triplication of all chromosomes except chromosome 18, the presence of Philadelphia (Ph) chromosome in 4-5 copies, and the presence of chromosome markers. LAMA-84 cells have morphological features of undifferentiated blast cells, but analyses have indicated that they belong to the megakaryocytic lineage; platelet peroxidase (PPO) was found in 8.5% of cells; LAMA-84 cells reacted spontaneously with poly- and monoclonal antibodies against the platelet glycoproteins (GP) IIb, IIIa, and the GPIIb/IIIa complex, whose presence was confirmed by crossed immunoelectrophoresis. LAMA-84 cells lack the membrane characteristics of lymphoid and mature granulocytic cells but do, however, react with certain antibodies to immature myeloid cells. Furthermore, they are positive with an antiglycophorin antibody, and contain alpha- and gamma-globin mRNA, thus demonstrating erythroid marker expression. Thus LAMA-84 is a tripotent, megakaryocytic, erythroid, and granulocytic cell line. The megakaryocytic and erythroid markers were enhanced by the addition of DMSO, butyrate, TPA, and hemin. The LAMA-84 cell line represents an interesting tool for the study of megakaryocytic and erythroid differentiation and the mechanisms of neoplastic growth.