MICROTUBULE-MOTOR ACTIVITY OF A YEAST CENTROMERE-BINDING PROTEIN COMPLEX
MICROTUBULE-MOTOR ACTIVITY OF A YEAST CENTROMERE-BINDING PROTEIN COMPLEX
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DOI：
10.1038/359533a0
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发表时间：
1992-10-08
期刊：
影响因子：
64.8
通讯作者：
CARBON, J
中科院分区：
文献类型：
--
作者：
HYMAN, AA;MIDDLETON, K;CARBON, J
在细胞分裂过程中，姐妹染色体在一种叫做有丝分裂纺锤体的微管结构上相互分离。蛋白质与着丝粒(染色体DNA的一个区域)结合形成着丝粒，动粒介导染色体与有丝分裂纺锤体微管的附着1，2。在萌芽酵母中，遗传分析表明125-bp着丝粒DNA序列(CEN序列)的28个碱基对(BP)CDEIII区是控制vivo3，4中染色体分离的主要区域。因此，在有丝分裂过程中，与CDEIII区结合的蛋白质可能将着丝粒与微管联系在一起。通过亲和层析分离出了一种与CDEIII DNA序列特异结合的蛋白质复合体(CBF3)。这里我们描述了动粒在体外的功能。CBF3复合体可以将DNA连接到微管，并且该复合体包含一个负端定向的微管马达。我们认为，在有丝分裂时，基于微管的马达形成了微管和染色体之间的基本联系。
DURING cell division, sister chromosomes segregate from each other on a microtubule-based structure called the mitotic spindle. Proteins bind to the centromere, a region of chromosomal DNA, to form the kinetochore, which mediates chromosome attachment to the mitotic spindle microtubules1,2. In the budding yeast Saccharomyces cerevisiae, genetic analysis has shown that the 28-base-pair (bp) CDEIII region of the 125-bp centromere DNA sequence (CEN sequence) is the main region controlling chromosome segregation in vivo3,4. Therefore it is likely that proteins binding to the CDEIII region link the centromeres to the microtubules during mitosis. A complex of proteins (CBF3) that binds specifically to the CDEIII DNA sequence has been isolated by affinity chromatography5. Here we describe kinetochore function in vitro. The CBF3 complex can link DNA to microtubules, and the complex contains a minus-end-directed microtubule-based motor. We suggest that microtubule-based motors form the fundamental link between microtubules and chromosomes at mitosis.