Endothelial nitric oxide synthase immunoreactivity in early gestation and in trophoblastic disease
Endothelial nitric oxide synthase immunoreactivity in early gestation and in trophoblastic disease
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DOI：
10.1136/jcp.51.6.427
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发表时间：
1998-06-01
影响因子：
3.4
通讯作者：
Shochina, M
中科院分区：
文献类型：
--
作者：
Ariel, I;Hochberg, A;Shochina, M
目的：研究内皮型一氧化氮合酶（eNOS）在正常胎盘中的定位，特别强调妊娠早期胎盘着床部位和妊娠滋养细胞疾病中滋养细胞不同亚型的eNOS定位。方法：应用抗eNOS抗体的免疫过氧化物酶技术，对妊娠早期和中期胎盘、胎盘植入、部分性和完全性葡萄胎以及绒毛膜癌。人胎盘催乳素（hPL）的免疫过氧化物酶染色进行平行sections.Results-Prominent免疫反应eNOS被发现存在于中间滋养层细胞的细胞柱的锚定绒毛和滋养层细胞在植入部位。染色也存在于合体滋养层，最明显的顶端细胞边界。在滋养层疾病，增殖大的单核细胞，这是强烈阳性的hPL，被发现是eNOS的免疫反应性。结论eNOS免疫反应性是强烈阳性的绒毛外滋养层细胞和在较小程度上在合体滋养层。在前者中，它可能在植入和血管侵袭中起作用。完全性葡萄胎和绒毛膜癌中分化为中间滋养细胞的细胞也显示出高水平的eNOS，这可能与滋养细胞疾病的血行传播模式有关。
Aims-To study the localisation of the endothelial nitric oxide synthase (eNOS) in the normal placenta, with special emphasis on the implantation site in the first trimester of pregnancy, and in the different subtypes of trophoblastic cells in gestational trophoblastic disease.Methods-The immunoperoxidase technique with an antibody directed against eNOS was applied to paraffin sections from first and second trimester placentas, placenta accreta, partial and complete hydatidiform moles, and choriocarcinoma. Immunoperoxidase staining for human placental lactogen (hPL) was performed on parallel sections.Results-Prominent immunoreactivity for eNOS was found to be present in the intermediate trophoblastic cells of the cell columns of the anchoring villi and in trophoblastic cells at the implantation site. Staining was also present in the syncytiotrophoblast, most conspicuous at the apical cell border. In trophoblastic disease, proliferating large mononuclear cells, which were strongly positive for hPL, were found to be immunoreactive for eNOS.Conclusions-eNOS immunoreactivity is strongly positive in the extravillous trophoblastic cells and to a lesser extent in the syncytiotrophoblast. In the former it may play a role in implantation and vascular invasion. Cells with differentiation to intermediate trophoblast in complete hydatidiform mole and choriocarcinoma also show high levels of eNOS, which may be associated with the haematogenous mode of spread of trophoblastic disease.