Glucose determination in fish plasma by two different moderate methods
Glucose determination in fish plasma by two different moderate methods
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DOI：
10.2754/avb201685040349
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发表时间：
2016-01-01
影响因子：
0.6
通讯作者：
Vojtek, Libor
中科院分区：
文献类型：
--
作者：
Bartonkova, Jana;Hyrsl, Pavel;Vojtek, Libor
硬骨鱼包括许多不同的物种，对经济和环境有很大的影响。葡萄糖是大多数脊椎动物的主要能量来源。鱼和哺乳动物都保持着特定的血糖水平，血糖水平受到多种因素的影响，如激素的产生、饮食和温度。因此，对于需要检查有机体当前状态的科学家来说，它是一个有用的指示器。目前最常用的测定养殖鱼血糖的方法是分光光度法，但这种方法往往不便于现场实验。在这里，我们报告了使用葡萄糖氧化酶(在实验室条件下使用)氧化血液中的β-D-葡萄糖的基础分光光度测定法可以很容易地被便携式血糖仪测量所取代，这种测量主要是为人体样本设计的。这种方法在实验室外使用的可能性和对样品体积的要求很小，因此非常有用。实地研究往往需要快速的样品处理，而且测量条件有限。使用血糖仪是一个简单而优雅的选择。我们用分光光度计法和人体血糖仪检测了74例丁卡锡(Tinca Tinca)的血浆样本(29名男性，45名女性)。我们的结果表明，两种方法之间存在显著的线性相关(P<；0.001)。本研究的主要贡献在于两种方法所得结果的可比性和互换性。
Teleost fish include many different species, having a large economic and environmental impact. Glucose is a key source of energy for most vertebrate organisms. Fish as well as mammals maintain a specific level of blood sugar -glycaemia, which is influenced by multiple factors such as hormone production, diet, and temperature. Therefore, it is a useful indicator for scientists who need to check the immediate state of an organism. The most frequently used method of measuring glycaemia in farmed fish is spectrophotometry; however, this method is often inconvenient for field experiments. Here we report that basic spectrophotometry using oxidation of blood beta D-glucose by glucose oxidase (used in laboratory conditions) can be easily substituted with portable glucometer measurements, primarily designed for human samples. The possibility of use outside the laboratory and its small sample volume demands make this method very useful. Field studies often require fast sample processing and conditions of measurement are limited. Using the glucometer is a simple and elegant option. We tested 74 blood plasma samples (29 males, 45 females) of tench (Tinca tinca) both spectrophotometrically and using the human glucometer. Our results show a significant linear correlation (P < 0.001) between both methods. The main contribution of this study consists in comparability and interchangeability of results obtained from both presented methods.