Nucleotide sequences of cDNAs coding for rat manganese-containing superoxide dismutase.
Nucleotide sequences of cDNAs coding for rat manganese-containing superoxide dismutase.
复制标题
编码大鼠含锰超氧化物歧化酶的 cDNA 的核苷酸序列。
DOI：
10.1093/nar/15.23.10070
复制
发表时间：
1987
影响因子：
14.9
通讯作者：
Crapo,JD
中科院分区：
文献类型：
--
作者：
Ho,YS;Crapo,JD
分子生物学实验室，变态反应、重症监护和呼吸医学部，医学系，杜克大学医学中心，达勒姆，NC 27710，美国提交1987年7月30日登录号Y 00497编码大鼠含锰超氧化物歧化酶（Mn SOD）的cDNA克隆已从构建于载体AgtII（Clontech Laboratories，股份有限公司、Palo Alto，CA）通过与人cDNA（图1中所示的序列）（1）交叉杂交。截短克隆的核苷酸序列在全长cDNA克隆的残基188和994之间是相同的，除了残基512处的C至G取代导致氨基酸残基143处的组氨酸被谷氨酰胺取代。这两个cDNA克隆的994位残基后的3 '非编码区的核苷酸序列不同。通过比较推导的氨基酸序列和已知的成熟人Mn SOD的氨基末端，初步翻译的前体的前24个氨基酸可能构成转运到线粒体的前导肽（2）。大鼠和人MnSOD的氨基酸序列与前导肽和成熟蛋白的同源性分别为89%和50%和93%
Laboratory of Molecular Biology, Division of Allergy, Critical Care and Respiratory Medicine, Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA Submitted July 30, 1987 Accession no. Y00497 cDNA clones coding for rat manganese-containing superoxide dismutase (Mn SOD) have been isolated from a Sprague-Dawley rat liver cDNA library constructed in vector Agtll (Clontech Laboratories, Inc., Palo Alto, CA) by cross hybridization with human cDNA (sequences shown in Figure 1)(1). The nucleotide sequence of a truncated clone is identical between residues 188 and 994 of the full-length cDNA clone except for a C to G substitution at residue 512 leading to substitution of histidine by glutamine at amino acid residue 143. The nucleotide sequences of the 3'noncoding region after residue 994 of these two cDNA clones are different. From comparing the deduced amino acid sequence and the known amino-terminal ofmature human Mn SOD, the first 24 amino acids of the primarily translated precursor may constitute the leader peptide for transportto the mitochondria (2). Amino acid sequences of the rat and human Mn SODs are 89% homologous with the leader peptides and mature proteins 50% and 93% homologouis, respectively