Internal Virus Polarization Model for Virus Retention by the Ultipor® VF Grade DV20 Membrane
Internal Virus Polarization Model for Virus Retention by the Ultipor® VF Grade DV20 Membrane
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DOI：
10.1002/btpr.1897
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发表时间：
2014-07-01
影响因子：
2.9
通讯作者：
Kuriyel, Ralf
中科院分区：
文献类型：
--
作者：
Jackson, Nigel B.;Bakhshayeshi, Meisam;Kuriyel, Ralf
最近的几项研究报告了病毒挑战过滤实验期间病毒截留率的下降，尽管不同过滤器的这种现象的控制机制仍不确定。进行实验以评价在恒压过滤期间通过Ultipor VF级DV 20病毒过滤器对PP 7和PR 772噬菌体的截留。虽然较大的PR 772噬菌体在所有条件下都被完全保留，但在高通量下观察到小的PP 7噬菌体的保留率下降2-log，即使在滤液通量没有下降的条件下也是如此。此外，用免疫球蛋白G溶液预污染膜对噬菌体保留没有影响。开发了内部极化模型来描述由噬菌体在过滤器内的上层（储库）中的积累引起的噬菌体保留的下降，这增加了对下层（排斥）的挑战。这种内部极化现象的独立支持提供了共聚焦显微镜的荧光标记的噬菌体膜内。该模型与大范围噬菌体滴度的噬菌体保留数据一致性良好，证实了病毒保留是通量依赖性的，并支持目前对病毒保留验证研究的建议。这些结果提供了重要的见解的病毒截留膜过滤器和其依赖于病毒过滤膜的底层结构的因素。(C)2014年美国化学工程师学会
Several recent studies have reported a decline in virus retention during virus challenge filtration experiments, although the mechanism(s) governing this phenomenon for different filters remains uncertain. Experiments were performed to evaluate the retention of PP7 and PR772 bacteriophage through Ultipor VF Grade DV20 virus filters during constant pressure filtration. While the larger PR772 phage was fully retained under all conditions, a 2-log decline in retention of the small PP7 phage was observed at high throughputs, even under conditions where there was no decline in filtrate flux. In addition, prefouling the membrane with an immunoglobulin G solution had no effect on phage retention. An internal polarization model was developed to describe the decline in phage retention arising from the accumulation of phage in the upper (reservoir) layer within the filter which increases the challenge to the lower (rejection) layer. Independent support for this internal polarization phenomenon was provided by confocal microscopy of fluorescently labeled phage within the membrane. The model was in good agreement with phage retention data over a wide range of phage titers, confirming that virus retention is throughput dependent and supporting current recommendations for virus retention validation studies. These results provide important insights into the factors governing virus retention by membrane filters and their dependence on the underlying structure of the virus filter membrane. (C) 2014 American Institute of Chemical Engineers