Tween 80 enhancing cellulase recyclability during activation of dissolving pulp
Tween 80 enhancing cellulase recyclability during activation of dissolving pulp
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Tween 80 增强溶解浆活化过程中纤维素酶的可回收性
DOI：
10.1080/02773813.2020.1722699
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发表时间：
2020-02-04
影响因子：
2
通讯作者：
Chen, Jiachuan
中科院分区：
文献类型：
--
作者：
Liu, Shanshan;Liu, Xiujing;Chen, Jiachuan
摘要提高纤维素酶的可回收性是降低溶解纸浆高酶成本的一条有希望的途径。为此，使用了两亲性表面活性剂吐温80来促进纤维素酶的回收。结果表明，纤维素酶-吐温复合体是通过疏水作用形成的(粒径增大了140%)。与对照相比，复合体对纤维素酶的最大结合容量和亲和力(即吸附能力)显著降低(分别为6.03vs24.3mgProtein/g和7.45vs13.8mlmlProtein/mg )。在添加吐温80的连续5个循环中，回收的纤维素酶活力比对照提高了65.7%。结果表明，回收的纤维素酶在粘度和反应活性方面都得到了提高。吐温80在纤维素酶回收中的积极作用支持了其他性质，如α-纤维素含量、碱溶度、分子量分布、形态和晶体结构。
Abstract Enhancing cellulase recyclability is a promising approach to reducing the high enzyme cost for activation of dissolving pulp. For this purpose, Tween 80, an amphiphilic surfactant, was employed to facilitate cellulase recovery from fibers. Results indicated that the Cellulase–Tween complex was formed (with a particle size increase of 140%) through a hydrophobic interaction. A much lower maximum binding capacity and affinity (i.e., absorption ability) of cellulase onto fibers (6.03 versus 24.3 mg protein/g cellulose and 7.45 versus 13.8 ml/mg protein) were achieved for the complex in comparison with the control. The recovered cellulase activity was increased up to 65.7% from the control one in five consecutive recycle rounds with Tween 80 addition. As a result, the recovered cellulase efficiency was improved in terms of viscosity and reactivity. Other properties, such as α-cellulose content, alkaline solubility, molecular weight distribution, morphology, and crystalline structure, were all supported by the positive effect of Tween 80 in cellulase recycling.