Antiproliferative and immunostimulatory activity of a protein from Pleurotus eryngii.
Antiproliferative and immunostimulatory activity of a protein from Pleurotus eryngii.
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DOI：
10.1002/jsfa.6665
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发表时间：
2014-12
影响因子：
4.1
通讯作者：
A. Mariga;Wen-jian Yang;Dorothy Kemuma Mugambi;Fei Pei;Liyan Zhao;Ya-ni Shao;Q. Hu
中科院分区：
文献类型：
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作者：
A. Mariga;Wen-jian Yang;Dorothy Kemuma Mugambi;Fei Pei;Liyan Zhao;Ya-ni Shao;Q. Hu
目前，蘑菇作为功能性食品、营养保健品或植物药物来源的使用已经增加。与此相反，可能的细胞毒性和免疫刺激活性的Pleumerynastatin（DC。不含法语）Quel蛋白（PEQP）未知。本文报道了PEQP的提取、体外抗肿瘤活性和免疫刺激活性。结果从杏鲍菇干燥子实体中提取、纯化并鉴定了PEQP。然后使用常规癌症药物（紫杉醇、多柔比星和丝裂霉素C）作为阳性对照，在人非小细胞肺癌A549（NSCLC）、胃腺癌BGC-823、肝细胞癌HepG 2和胃癌HGC-27细胞系中评价其体外抗增殖活性。所获得的蛋白级分（PEQP 1、2、3和4）剂量依赖性地抑制肿瘤细胞增殖，其中级分PEQP 2在所有肿瘤细胞中具有显著（P < 0.05）毒性。PEQP对正常肝Chang细胞无明显毒性作用，而丝裂霉素C可明显抑制其增殖。此外，PEQP剂量依赖性地刺激RAW 264.7细胞系的增殖、溶酶体酶活性、胞饮、一氧化氮和过氧化氢的产生。结论该蛋白作为一种具有免疫刺激活性的天然抗肿瘤药物，在功能性食品中具有潜在的应用价值。
BACKGROUND Currently, the use of mushrooms as functional foods, nutraceuticals or phytopharmaceuticals source has risen. In contrast, the possible cellular cytotoxicity and immunostimulatory activity of Pleurotus eryngii (DC. ex Fr.) Quel protein (PEQP) is unknown. Here we report extraction, anti-tumorigenic and immunostimulatory activity of PEQP in vitro. RESULTS PEQP was extracted from the dried fruiting bodies of P. eryngii, purified and characterised. Its in vitro antiproliferative activity was then evaluated in human non-small cell lung cancer A549 (NSCLC), stomach adenocarcinoma BGC-823, hepatocellular carcinoma HepG2 and gastric carcinoma HGC-27 cell lines using conventional cancer drugs (paclitaxel, doxorubicin and mitomycin C) as positive controls. The protein fractions (PEQP 1, 2, 3 and 4) obtained inhibited tumour cell proliferation dose-dependently with fraction PEQP 2 having significant (P < 0.05) toxicity in all tumour cells. PEQP had no significant toxicity on normal liver Chang cells but their proliferation was significantly inhibited by mitomycin C. Moreover, PEQP stimulated the proliferation, lysosomal enzyme activity, pinocytosis, nitric oxide and hydrogen peroxide production of RAW 264.7 cell lines dose-dependently. CONCLUSION Based on these results, P. eryngii protein has a potential application in functional foods as a natural anti-tumour agent with immunostimulatory activity.