rRNA probes used to quantify the effects of glycomacropeptide and α-lactalbumin supplementation on the predominant groups of intestinal bacteria of infant rhesus monkeys challenged with enteropathogenic Escherichia coli
rRNA probes used to quantify the effects of glycomacropeptide and α-lactalbumin supplementation on the predominant groups of intestinal bacteria of infant rhesus monkeys challenged with enteropathogenic Escherichia coli
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DOI：
10.1097/00005176-200309000-00014
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发表时间：
2003-09-01
影响因子：
2.9
通讯作者：
Lönnerdal, B
中科院分区：
文献类型：
--
作者：
Brück, WM;Kelleher, SL;Lönnerdal, B
目的：某些乳汁因子可能有助于促进宿主友好的结肠微生物群（例如双歧杆菌、乳酸杆菌）的生长，并解释为什么母乳喂养的婴儿比配方奶喂养的婴儿经历更少且更轻微的肠道感染。本研究调查了在配方奶粉中添加两种此类乳因子的效果。 材料和方法：对幼年恒河猴进行母乳喂养、对照配方奶粉或添加糖巨肽 (GMP) 或 α-乳糖素 (α-LA) 的配方奶，从出生到 5 个月大。每月抽血并每周收集直肠拭子。在 4.5 个月大时，口服 10(8) 个菌落形成单位的肠致病性大肠杆菌 O127，菌株 2349/68 (EPEC)，并评估对感染的反应。通过独立于培养物的荧光原位杂交测定感染前后直肠拭子的细菌学。结果：攻击后，母乳喂养的婴儿和喂养添加 α-LA 的配方奶粉的婴儿没有腹泻，而喂养添加 GMP 的配方奶粉的婴儿有间歇性腹泻。对照组婴儿出现急性腹泻。结论：婴儿配方奶粉中添加适当的乳蛋白可能有助于提高婴儿抵抗大肠杆菌引起的急性感染的能力。
Objectives: Certain milk factors may help to promote the growth of a host-friendly colonic microflora (e.g. bifidobacteria, lactobacilli) and explain why breast-fed infants experience fewer and milder intestinal infections than those who are formula-fed. The effects of supplementation of formula with two such milk factors was investigated in this study.Materials and Methods: Infant rhesus macaques were breastfed, fed control formula, or formula supplemented with glycomacropeptide (GMP) or alpha-lactalburnin (alpha-LA) from birth to 5 months of age. Blood was drawn monthly and rectal swabs were collected weekly. At 4.5 months of age, 10(8) colonyforming units of enteropathogenic E.coli O127, strain 2349/68 (EPEC) was given orally and the response to infection assessed. The bacteriology of rectal swabs pre- and post-infection was determined by culture independent fluorescence in situ hybridization.Results: Post-challenge, breast-fed infants and infants fed alpha-LA-supplemented formula had no diarrhea, whilst those infants fed GMP-supplemented formula had intermittent diarrhea. In infants fed control formula the diarrhea was acute.Conclusions: Supplementation of infant formula with appropriate milk proteins may be useful for improving the infant's ability to resist acute infection caused by E.coli.