The use of fluorescein-labelled lectins in the detection and identification of fungi pathogenic for man: a preliminary study.
The use of fluorescein-labelled lectins in the detection and identification of fungi pathogenic for man: a preliminary study.
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使用荧光素标记的凝集素检测和鉴定对人类致病的真菌：初步研究。
DOI：
10.1099/00222615-11-3-315
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发表时间：
1978
影响因子：
3
通讯作者：
B. Herbertson
中科院分区：
文献类型：
--
作者：
R. W. Stoddart;B. Herbertson
荧光素标记的凝集素已知的不同糖的特异性被用于在外科手术和死后材料的石蜡切片中鉴定真菌。用五种荧光素标记的凝集素研究了烟曲霉、皮炎芽孢菌、白色念珠菌、新型隐球菌、巴西副球虫和米根霉，并对它们与这些凝集素的反应差异进行了研究。结果与已知的生物化学非常一致，该方法为执业组织病理学家提供了希望。
Fluorescein-labelled lectins of known specificities for different sugars were used in an attempt to identify fungi in paraffin sections of surgical and post-mortem material. Aspergillus fumigatus, Blastomyces dermatitidis, Candida albicans, Cryptococcus neoformans, Paracoccidioides brasiliensis and Rhizopus oryzae have been studied with five fluorescein-labelled lectins and with basis of differences in their reactions with these stains. The results accord well with what is known of the chemistry of the organisms and the method offers promise to practising histopathologists.