Nucleic acid and protein sequences of phosphocholine-binding light chains.
Nucleic acid and protein sequences of phosphocholine-binding light chains.
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DOI：
10.1084/jem.153.5.1366
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发表时间：
1981-05-01
期刊：
影响因子：
--
通讯作者：
Scharff MD
中科院分区：
文献类型：
--
作者：
Kwan SP;Rudikoff S;Seidman JG;Leder P;Scharff MD
从细胞总DNA中克隆出含有S107小鼠免疫球蛋白轻链可变区和恒定区序列编码的18千碱基DNA片段。报道了kappa链可变区基因的完整核苷酸序列。DNA编码的氨基酸序列通过残基88与T15轻链的蛋白序列一致。直接序列分析证实J1连接段参与了产生活性kappa轻链基因的重组事件。
An 18-kilobase DNA fragment containing the sequence coding for both the variable and constant regions of the S107 mouse immunoglobulin light chain was cloned from total cellular DNA. The complete nucleotide sequence of the kappa-chain variable-region gene is reported. Determination of the amino acid sequence encoded by the DNA is found to be identical to the protein sequence of the T15 light chain through residue 88. Direct sequence analysis confirmed that the J1 joining segment is used in the recombination event producing the active kappa light chain gene.