THE LOCALIZATION OF ALBUMIN AND FIBRINOGEN IN HUMAN LIVER CELLS.
THE LOCALIZATION OF ALBUMIN AND FIBRINOGEN IN HUMAN LIVER CELLS.
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DOI：
10.1083/jcb.20.2.271
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发表时间：
1964-02
期刊：
影响因子：
--
通讯作者：
COONS AH
中科院分区：
文献类型：
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作者：
HAMASHIMA Y;HARTER JG;COONS AH
人肝切片用抗人血清白蛋白和/或抗人纤维蛋白单体荧光结合物染色。大约10%的肝细胞对人血清白蛋白进行特殊染色，1%对纤维蛋白原进行染色，0.1%对两者都进行染色。大约18%的Kupffer细胞对人血清白蛋白进行了特殊染色，33%对纤维蛋白原进行了染色。两种细胞的染色主要是胞浆，但根据固定方法的不同，在一些实质细胞的细胞核中也发现了白蛋白。在面对胆小管的细胞膜内可以看到比1%的实质细胞更多的纤维蛋白原的胞浆颗粒，呈弥漫性胞浆染色。讨论了制备抗这些抗原的荧光偶联物和固定这些抗原用于免疫荧光染色的技术难点。
Human liver sections were stained with anti-human serum albumin and/or anti-human fibrin monomer fluorescent conjugates. Approximately 10 per cent of the hepatic cells stained specifically for human serum albumin,1 per cent for fibrinogen, and 0.1 per cent for both. Approximately 18 per cent of the Kupffer cells stained specifically for human serum albumin and 33 per cent for fibrinogen. Staining of both cell types was mainly cytoplasmic, although albumin was found in the nuclei of some parenchymal cells, depending on the method of fixation. Cytoplasmic granules staining specifically for fibrinogen could be seen just inside the cell membrane facing the bile caniculi in many more parenchymal cells than the 1 per cent showing diffuse cytoplasmic staining. The technical difficulties involved in preparing fluorescent conjugates against these antigens and in the fixation of these antigens for immunofluorescent staining are discussed.