Highly stabilized lipase in polyaniline nanofibers for surfactant-mediated esterification of ibuprofen.
Highly stabilized lipase in polyaniline nanofibers for surfactant-mediated esterification of ibuprofen.
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DOI：
10.1021/la404189e
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发表时间：
2014-01
期刊：
影响因子：
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通讯作者：
Sung-Gil Hong;H. Kim;Jungbae Kim
中科院分区：
文献类型：
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作者：
Sung-Gil Hong;H. Kim;Jungbae Kim
通过吸附、沉淀和交联三步反应，将假丝酵母脂肪酶（LP）固定在聚苯胺纳米纤维（PANFs）中，形成最终的固定物“EAPC”。与酶吸附法（EA）和酶吸附交联法（EAC）相比，EAPC的固定化活性分别提高了5.1倍和5.9倍。在震荡（200 rpm）的缓冲液中孵育84天后，EAPC保持了74%的初始活性，而EA和EAC分别保持了11%和24%的初始活性。采用稳定、高效的EAPC在异辛烷中催化S-(+)-布洛芬与1-丙醇酯化反应分离外消旋布洛芬。在反应介质中加入100 mM的琥珀酸二辛酯（AOT），酯化活性提高了61倍，这可以解释为EAPC在异辛烷中的分散性较好。102 h后，EAPC对外消旋布洛芬的酯化反应转化率为42%，而在相同条件下，EA和EAC的转化率分别为1.2%和1.4%。EAPC方法增加了LP的负载和稳定性，并且EAPC与表面活性剂的组合可以用于有机溶剂中的高效酶促反应。
Lipase (LP) from Candida rugosa was immobilized and stabilized in polyaniline nanofibers (PANFs) via a three-step process of enzyme adsorption, precipitation, and cross-linking, which generates the final immobilization called "EAPC". The activity of EAPC was 5.1 and 5.9 times higher than those of LP immobilizations via enzyme adsorption (EA) and enzyme adsorption/cross-linking (EAC), respectively. After incubation in an aqueous buffer under shaking (200 rpm) for 84 days, EAPC maintained 74% of its initial activity, while EA and EAC retained 11 and 24% of their initial activities, respectively. Highly stable and active EAPC was employed for the resolution of racemic ibuprofen via esterification of S-(+)-ibuprofen with 1-propanol in isooctane. The addition of 100 mM dioctyl sulfosuccinate (AOT) into the reaction medium increased the esterification activity by 61-fold, which can be explained by the better dispersion of EAPC in isooctane. EAPC showed 42% conversion in the esterification of racemic ibuprofen after 102 h, whereas EA and EAC showed only 1.2 and 1.4% conversion in the same condition, respectively. The EAPC approach increases both loading and stability of LP, and the combination of EAPC with the surfactant addition can be employed for efficient enzymatic reactions in organic solvents.