Protection against Experimental Melioidosis following Immunization with Live Burkholderia thailandensis Expressing a manno-Heptose Capsule
Protection against Experimental Melioidosis following Immunization with Live Burkholderia thailandensis Expressing a manno-Heptose Capsule
复制标题
DOI：
10.1128/cvi.00113-13
复制
发表时间：
2013-07-01
影响因子：
--
通讯作者：
Atkins, Timothy P.
中科院分区：
文献类型：
--
作者：
Scott, Andrew E.;Laws, Thomas R.;Atkins, Timothy P.
类鼻疽是由类鼻疽伯克霍尔德杆菌引起的严重传染病。它对抗生素治疗具有高度耐药性，目前还没有获得许可的疫苗。泰国伯克霍尔德氏菌是类鼻疽伯克霍尔德氏菌的近亲，但对哺乳动物基本上无毒。在本报告中，我们详细介绍了活体泰国伯克利氏菌 E555 免疫的保护功效，该菌株已被证明表达与类鼻疽伯克氏菌类似的抗原荚膜。在小鼠感染模型中，E555 免疫诱导了针对致命性腹膜内类鼻疽杆菌攻击的显着保护作用，在研究过程中，即使接受高达 6,000 中位致死剂量的攻击，也没有小鼠死于感染。相比之下，用不表达类鼻疽杆菌样荚膜的泰国芽孢杆菌免疫的小鼠的保护水平显着降低。 E555 免疫小鼠的 IgG 水平显着高于用非荚膜泰国芽孢杆菌免疫的小鼠，并且这些抗体反应主要针对荚膜。
Melioidosis is a severe infectious disease caused by Burkholderia pseudomallei. It is highly resistant to antibiotic treatment, and there is currently no licensed vaccine. Burkholderia thailandensis is a close relative of Burkholderia pseudomallei but is essentially avirulent in mammals. In this report, we detail the protective efficacy of immunization with live B. thailandensis E555, a strain which has been shown to express an antigenic capsule similar to that of B. pseudomallei. Immunization with E555 induced significant protection against a lethal intraperitoneal B. pseudomallei challenge in a mouse model of infection, with no mice succumbing to infection over the course of the study, even with challenges of up to 6,000 median lethal doses. By comparison, mice immunized with B. thailandensis not expressing a B. pseudomallei-like capsule had significantly decreased levels of protection. E555-immunized mice had significantly higher levels of IgG than mice immunized with noncapsulated B. thailandensis, and these antibody responses were primarily directed against the capsule.