Combination Therapy Using Human Adipose-derived Stem Cells on the Cavernous Nerve and Low-energy Shockwaves on the Corpus Cavernosum in a Rat Model of Post-prostatectomy Erectile Dysfunction
Combination Therapy Using Human Adipose-derived Stem Cells on the Cavernous Nerve and Low-energy Shockwaves on the Corpus Cavernosum in a Rat Model of Post-prostatectomy Erectile Dysfunction
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DOI：
10.1016/j.urology.2015.10.021
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发表时间：
2016-02-01
期刊：
影响因子：
2.1
通讯作者：
Lee, Ji Youl
中科院分区：
文献类型：
--
作者：
Jeon, Seung Hwan;Shrestha, Kshitiz Raj;Lee, Ji Youl
目的探讨人脂肪源性干细胞（h-ADSCs）治疗海绵体神经损伤与低能量冲击波治疗海绵体对前列腺切除术后勃起功能障碍大鼠模型的联合治疗效果。材料与方法将大鼠随机分为5组：对照组、双侧海绵体神经损伤（BCNI）组、脂肪源性干细胞（ADSC）组（BCNI组，海绵体上有h-ADSCs）、SWT组（BCNI组，海绵体上有低能SWT）、ADSC/SWT组（BCNI组，h-ADSCs与低能SWT联合）。4周后，用海绵体内压力评估勃起功能。通过免疫染色、免疫印迹和环鸟苷单磷酸测定来评估海绵体神经和阴茎组织。结果与其他实验组相比，ADSC/SWT显著改善了海绵体内压力。ADSC显著提高海绵体神经β - iii微管蛋白的表达，SWT显著提高海绵体血管内皮生长因子的表达。ADSC/SWT组与单独使用ADSC或SWT组相比，α -平滑肌肌动蛋白含量（P < 0.05）、阴茎背神经一氧化氮合酶（nNOS）、内皮一氧化氮合酶（eNOS）蛋白表达（P < 0.05）和环鸟苷单磷酸水平（P < 0.05）均显著升高。此外，ADSC/SWT可降低海绵体的凋亡指数。结论在本研究中，h-ADSCs对损伤海绵体神经的恢复有作用，低能量SWT促进海绵体血管生成。h-ADSCs联合低能量SWT对前列腺切除术后勃起功能障碍患者的勃起功能恢复有良好的作用。(C) 2016 Elsevier Inc.
OBJECTIVE To investigate combined therapeutic efficacy of human adipose-derived stem cells (h-ADSCs) application on injured cavernous nerve and low-energy shockwave therapy (SWT) on the corpus cavernosum in a rat model of post-prostatectomy erectile dysfunction.MATERIALS AND METHODS Rats were randomly divided into 5 groups: control, bilateral cavernous nerve injury (BCNI), adipose-derived stem cell (ADSC) (BCNI group with h-ADSCs on the cavernous nerve), SWT (BCNI group with low-energy SWT on the corpus cavernosum), and ADSC/SWT (BCNI group with a combination of h-ADSCs and low-energy SWT). After 4 weeks, erectile function was assessed using intracavernosal pressure. The cavernous nerves and penile tissue were evaluated through immunostaining, Western blotting, and a cyclic guanosine monophosphate assay.RESULTS ADSC/SWT significantly improved intracavernosal pressure compared to the other experimental group. ADSC had significantly increased beta-III tubulin expression of the cavernous nerve, and SWT had a markedly enhanced vascular endothelial growth factor expression in corpus cavernosum. The ADSC/SWT group had a significantly increased in alpha smooth muscle actin content (P < .05), neural nitric oxide synthase (nNOS) of the dorsal penile nerve (P < .05), endothelial nitric oxide synthase (eNOS) protein expression (P < .05), and cyclic guanosine monophosphate level (P < .05) compared to the ADSC or SWT alone group. In addition, ADSC/SWT reduces the apoptotic index in the corpus cavernosum.CONCLUSION In this study, h-ADSCs showed an effect on the recovery of injured cavernous nerve and low-energy SWT improved angiogenesis in the corpus cavernosum. The h-ADSCs combined with low-energy SWT showed beneficial effect on the recovery of erectile function in postprostatectomy erectile dysfunction. (C) 2016 Elsevier Inc.