Human menstrual blood-derived stem cells promote the repair of impaired endometrial stromal cells by activating the p38 MAPK and AKT signaling pathways
Human menstrual blood-derived stem cells promote the repair of impaired endometrial stromal cells by activating the p38 MAPK and AKT signaling pathways
复制标题
人经血干细胞通过激活 p38 MAPK 和 AKT 信号通路促进受损子宫内膜基质细胞的修复。
DOI：
10.1016/j.repbio.2018.06.003
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发表时间：
2018-09-01
影响因子：
2.1
通讯作者：
Zhang, Songying
中科院分区：
文献类型：
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作者：
Zhu, Haiyan;Jiang, Yinshen;Zhang, Songying
多项研究证实，人经血干细胞(MenSCs)在再生医学或细胞治疗中具有潜在的应用前景。然而，目前尚不清楚MenSCs在子宫内膜修复中的作用。我们评估了MenSCs对受损的子宫内膜间质细胞(ESCs)的保护作用，以及参与这一过程的信号通路。米非司酮用于损伤人ESCs，随后将其与MenSCs共同培养。检测ESCs的增殖、凋亡和迁移，以及相关信号蛋白的表达，包括总p38丝裂原活化蛋白激酶、P-p38、总蛋白激酶B(AKT)、P-AKT、β-连环蛋白和血管内皮生长因子(VEGF)。MenSCs明显恢复受损ESCs的增殖和迁移能力，抑制ESC的凋亡，上调P-AKT、P-p38、VEGF和β-catenin的蛋白表达。我们的发现表明，基于MenSC的治疗可能是治疗子宫内膜损伤的有前途的策略，AKT和p38信号通路可能参与了这一过程。
Multiple studies have confirmed that human menstrual blood-derived stem cells (MenSCs) have potential applications in regenerative medicine or cell therapy. However, the contribution of MenSCs to endometrial repair is currently unknown. We evaluated the protective effects of MenSCs on impaired endometrial stromal cells (ESCs), as well as the signaling pathways involved in this process. Mifepristone was used to damage human ESCs, which were subsequently cocultured with MenSCs. The proliferation, apoptosis, and migration of ESCs were assessed, together with the expression of related signaling proteins including total p38 mitogen-activated protein kinase, P-p38, total protein kinase B (AKT), P-AKT, beta-catenin, and vascular endothelial growth factor (VEGF). MenSCs significantly recovered the proliferation and migration ability of impaired ESCs, inhibited ESC apoptosis, and upregulated protein expression of P-AKT, P-p38, VEGF, and beta-catenin. Our findings suggest that MenSC-based therapies could be promising strategies for the treatment of endometrial injury, and that AKT and p38 signaling pathways may be involved in this process.