Ultrastructure, SSU rRNA Gene Sequences and Phylogenetic Relationships of Flamella Schaeffer, 1926 (Amoebozoa), with Description of Three New Species
Ultrastructure, SSU rRNA Gene Sequences and Phylogenetic Relationships of Flamella Schaeffer, 1926 (Amoebozoa), with Description of Three New Species
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DOI：
10.1016/j.protis.2008.09.004
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发表时间：
2009-02-01
期刊：
影响因子：
2.5
通讯作者：
Michel, Rolf
中科院分区：
文献类型：
--
作者：
Kudryavtsev, Alexander;Wylezich, Claudia;Michel, Rolf
我们分离并描述了三个新的淡水变形虫种，可以明确地分配到属Flamella Schaeffer，1926年通过光学显微镜。Flamella属在变形虫中的系统发育位置尚不清楚，也缺乏基因序列数据。我们对五种Flamella spp.的SSU rRNA基因进行了测序，包括先前描述的F.埃及Michel et Smirnov，1999年。系统发育树表明，小鞭毛藻属是变形虫门内的单系分支。它属于包括丝状阿米巴属的分支，“蜘蛛属”，一些未鉴定或未培养的真核生物的原中柱和几个SSU rRNA序列。这支分支一贯分支接近Archamoeulae，Mycetozoa，Acramoeba dendroida和Multicilia marina;与以前的假设相反，Flamella spp.没有显示出任何相关性，无论是Leptomyxida，或Flabellinea。营养型变形虫的超微结构，特别是孢囊的研究表明相当相似的Comandonia operculata Pernin et Pussard，1979年。因此，我们建议，Comandonia可能是一个初级的同义词Flamella，虽然更多的超微结构数据有关Comandonia operculata是必要的，以检验这一假设。(C)2008年Elsevier GmbH。All rights reserved.
We isolated and described three new freshwater amoebozoan species that could be unambiguously assigned to the genus Flamella Schaeffer, 1926 by light microscopy. The phylogenetic position of the genus Flamella within the Amoebozoa was unknown, and gene sequence data were lacking. We sequenced the SSU rRNA gene of five Flamella spp., including a previously described F. aegyptia Michel et Smirnov, 1999. The phylogenetic trees inferred from these data showed, that Flamella is monophyletic and robustly branches within Amoebozoa. It belongs to a clade comprising Filamoeba spp., "Arachnula" sp., some protostelids and several SSU rRNA sequences of unidentified or uncultured eukaryotes. This clade consistently branched close to Archamoebae, Mycetozoa, Acramoeba dendroida and Multicilia marina; in contrast to the previous hypotheses, Flamella spp. did not show any relatedness either to Leptomyxida, or to Flabellinea. The ultrastructure of trophic amoebae and especially cysts of the species studied showed considerable similarity to Comandonia operculata Pernin et Pussard, 1979. We therefore suggest that Comandonia may be a junior synonym of Flamella, although more ultrastructural data about Comandonia operculata are necessary to test this hypothesis. (C) 2008 Elsevier GmbH. All rights reserved.