Brassica napusGLABRA3-1 promotes anthocyanin biosynthesis and trichome formation in true leaves when expressed in Arabidopsis thaliana
Brassica napusGLABRA3-1 promotes anthocyanin biosynthesis and trichome formation in true leaves when expressed in Arabidopsis thaliana
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DOI：
10.1111/plb.12633
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发表时间：
2018-01-01
期刊：
影响因子：
3.9
通讯作者：
Chen, M.
中科院分区：
文献类型：
--
作者：
Gao, C.;Guo, Y.;Chen, M.
已有研究表明，拟南芥中的一个bHLH转录因子GLABRA 3（AtGL 3）在花色素苷合成和毛状体形成中起着重要作用。然而，还没有这样的研究同源物，BnGL 3，从密切相关的作物，甘蓝型油菜。本文分析了B.napus品种秦油七号BnGL 3 -1编码区序列，鉴定了其保守蛋白结构域，并进行了系统发育分析，以阐明其与一系列植物同源物的关系。当BnGL 3 - 1作为与绿色荧光蛋白的融合蛋白在烟草叶片中表达时，BnGL 3 -1在细胞核中积累，与其作为转录因子的预测功能一致。拟南芥gl 3 -3突变体中BnGL 3 -1基因的异位表达导致真叶中的花青素苷水平和毛状体数量高于野生型植物。此外，BnGL 3 -1在gl 3 -3中的过表达分别补偿了参与花色素苷生物合成和毛状体形成的基因的促进和抑制，这已经在gl 3 -3幼枝和扩展的真叶中报道。这项研究提供了新的见解GL 3花青素的生物合成和毛状体的形成在十字花科植物的功能，并代表了一个有前途的目标，油菜的遗传操作。
Previous studies have shown that GLABRA3 (AtGL3), a bHLH transcription factor, plays essential roles in anthocyanin biosynthesis and trichome formation in Arabidopsis thaliana. However, there have been no such studies of a homologue, BnGL3, from the closely related crop, Brassica napus. Here, we analysed the BnGL3-1 coding domain sequence from the B.napus cultivar QINYOU Seven, identified conserved protein domains and performed a phylogenetic analysis to elucidate its relationship with homologues form a range of plant species. When expressed in tobacco leaves as a fusion protein with green fluorescent protein, BnGL3-1 accumulated in the nucleus, consistent with its predicted function as a transcription factor. Ectopic expression of the BnGL3-1 gene in the A.thaliana gl3-3 mutant resulted in levels of anthocyanins and numbers of trichomes in true leaves that were higher than in wild-type plants. Moreover, overexpression of BnGL3-1 in gl3-3 compensated for the promotion and repression of genes involved in anthocyanin biosynthesis and trichome formation, respectively, that has been reported in gl3-3 young shoots and expanding true leaves. This study provides new insights into GL3 function in anthocyanin biosynthesis and trichome formation in crucifers, and represents a promising target for genetic manipulation of B.napus.