Dynamic expression of Runx1 in skin affects hair structure
Dynamic expression of Runx1 in skin affects hair structure
复制标题
DOI：
10.1016/j.mod.2006.08.002
复制
发表时间：
2006-11-01
影响因子：
2.6
通讯作者：
Gat, Uri
中科院分区：
文献类型：
--
作者：
Raveh, Eli;Cohen, Shulamit;Gat, Uri
三种哺乳动物 Runx 转录因子（其中一些已知与人类遗传疾病和癌症有关）是胚胎发育的关键参与者，并且是细胞命运决定和器官发生的关键调节因子。在这里，我们报告了 Runx1 在小鼠毛发和其他皮肤附属物发育过程中的表达，并描述了 Runx1 对结构毛发输出的影响。在表达三种 Runx 蛋白的毛囊中，Runx1 的表达在间充质和上皮区室中最为突出。上皮表达包括形成毛干和凸起层的毛发角蛋白，有趣的是，Runx1 与角蛋白 15（一种假定的毛囊干细胞标记物）共表达。在毛发间充质中，在毛发形态发生的早期阶段，RunxI 在离散的真皮上皮下层中表达，而在后期阶段，它在真皮乳头中以毛发周期依赖性模式表达。为了阐明 Runx1 在毛囊中的功能，我们生成了 Runx1 表皮条件敲除模型，发现突变小鼠表现出显着的锯齿状毛发类型的结构变形。这些数据将 RunxI 描述为几种毛囊细胞类型的新型特异性标记物，并阐明了其在毛发形态发生和分化中的作用。 (c) 2006 Elsevier Ireland Ltd. 保留所有权利。
The three mammalian Runx transcription factors, some of which are known to be involved in human genetic diseases and cancer, are pivotal players in embryo development and function as key regulators of cell fate determination and organogenesis. Here, we report the expression of Runx1 during the development of hair and other skin appendages in the mouse and describe the effect of Runx1 on the structural hair output. in hair follicles, where the three Runx proteins are expressed, Runx1 expression is most prominent in both mesenchymal and epithelial compartments. The epithelial expression includes the hair keratin forming layers of the hair shaft and the bulge, where interestingly, Runx1 is co-expressed with keratin 15, a putative hair follicle stem cell marker. In the hair mesenchyme, during early stages of hair morphogenesis, RunxI is expressed in a discrete dermal sub-epithelial layer, while at later stages it is found in a hair cycle dependent pattern in the dermal papilla. To elucidate the function of Runx1 in the hair follicle we have generated a Runx1 epidermal conditional knockout and found that the mutant mice display a remarkable structural deformation of the zigzag hair type. The data delineate RunxI as a novel specific marker of several hair follicle cell types and sheds light on its role in hair morphogenesis and differentiation. (c) 2006 Elsevier Ireland Ltd. All rights reserved.