Non-invasive assessment of placental perfusion in vivo using arterial spin labeling (ASL) MRI: A preclinical study in rats
Non-invasive assessment of placental perfusion in vivo using arterial spin labeling (ASL) MRI: A preclinical study in rats
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DOI：
10.1016/j.placenta.2019.01.019
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发表时间：
2019-02-01
期刊：
影响因子：
3.8
通讯作者：
Siauve, Nathalie
中科院分区：
文献类型：
--
作者：
Deloison, Benjamin;Salomon, Laurent J.;Siauve, Nathalie
简介：胎盘灌注的无创评估对于临床实践中表征胎盘功能具有重要意义。本文提出了一种使用 ASL 的严格非侵入性 MRI 技术来量化体内胎盘血流量。本研究的目的是开发一种功能磁共振成像工具，通过在 4.7 T 下使用动脉自旋标记 (ASL) MRI 来量化大鼠胎盘血流量 (PBF)。 材料和方法：使用小动物专用磁铁，在 22 天妊娠期的第 20 天对怀孕大鼠进行 MRI。 Look-Locker 流敏交替反转恢复梯度回波序列被开发为 ASL 技术（TE：1.55 ms；TR：3.5 ms、TI：56 ms、deltaTI：56 ms、FA：20 度、矩阵：128x128、8 段、4 Nex）。通过全局和切片选择性反演进行标记，并获得每种反演模式的 T1 图。然后从全局反转和切片选择性反转之间 T1 变化的区室模型中得出 PBF。结果：完整的协议已完成，ASL 图像后处理在 18 只大鼠中成功。对 47 个胎盘进行了分析，平均 PBF 为 147 +/- 70 ml/min/100 g 胎盘，与已发表的使用侵入性技术的胎盘灌注值一致。结论：ASL MRI 对于 4.7 T 大鼠的 PBF 定量是可行的。该技术不需要使用造影剂，可能对非侵入性纵向和体内动物研究产生影响，并可能对人类管理有用。怀孕。
Introduction: Non-invasive assessment of placental perfusion is of great interest to characterize placental function in clinical practice. This article proposes a strictly non-invasive MRI technique using ASL to quantify placental blood flow in vivo. The aim of this study was to develop a fMRI tool to quantify placental blood flow (PBF) in rat, by using arterial spin labeling (ASL) MRI at 4.7 T.Materials and methods: MRI was performed with a dedicated magnet for small animals, in pregnant rats on day 20 of the 22-day gestation period. A Look-Locker flow-sensitive alternating inversion recovery gradient echo sequence was developed as ASL technique (TE: 1.55 ms; TR: 3.5 ms, TI: 56 ms, deltaTI: 56 ms, FA: 20 degrees, Matrix: 128x128, 8 segments, 4 Nex). Labeling was performed with global and slice-selective inversions, and T1 map was obtained for each mode of inversion. PBF was then derived from a compartmental model of the variation of T1 between global and slice-selective inversions.Results: The full protocol was completed and ASL image post-processing was successful in 18 rats. Forty-seven placentas were analyzed, with a mean PBF of 147 +/- 70 ml/min/100 g of placenta, consistent with published values of placental perfusion using invasive techniques.Conclusion: ASL MRI is feasible for the quantification of PBF in rats at 4.7 T. This technique, which requires no administration of contrast media, could have implications for non-invasive longitudinal and in vivo animal studies and may be useful for the management of human pregnancies.