Isolation of a mouse MT2‐MMP gene from a lung cDNA library and identification of its product
Isolation of a mouse MT2‐MMP gene from a lung cDNA library and identification of its product
复制标题
从肺cDNA文库中分离小鼠MT2-MMP基因并鉴定其产物
DOI：
10.1016/s0014-5793(96)01537-2
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发表时间：
1997
期刊：
影响因子：
3.5
通讯作者：
M. Seiki
中科院分区：
文献类型：
--
作者：
Masaaki Tanaka;Hiroshi Sato;T. Takino;K. Iwata;M. Inoue;M. Seiki
We have isolated a new MT‐MMP related gene of 3.3 kb from a mouse lung cDNA library using a humanMT1‐MMPcDNA as a probe. The deduced protein sequence shows 87% homology to human MT2‐MMP and 52, 50 and 29% to MT1‐MMP, MT3‐MMP and MT4‐MMP, respectively. Thus the gene is thought to be a mouse homologue of humanMT2‐MMP. A monoclonal antibody raised against a synthetic peptide recognized mouse MT2‐MMP as a 70 kDa protein. Like MT1‐ and MT3‐MMPs, mouse MT2‐MMP caused activation of pro‐gelatinase A upon co‐transfection into COS‐1 cells. © 1997 Federation of European Biochemical Societies. All rights reserved
