Diffuse mucosal inflammation in nasal polyps and adjacent middle turbinate
Diffuse mucosal inflammation in nasal polyps and adjacent middle turbinate
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DOI：
10.1016/j.otohns.2005.09.026
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发表时间：
2006-02-01
影响因子：
3.4
通讯作者：
Wang, DY
中科院分区：
文献类型：
--
作者：
Hao, J;Pang, YT;Wang, DY
背景技术背景：鼻息肉（Nasal Polypp，NP）是鼻内最常见的肿块性病变，其病因和发病机制尚不清楚。目的：探讨亚洲鼻息肉患者的细胞炎症类型与变态反应的关系。用一组抗体进行免疫组织化学染色，用于CD 4+和CD 8 + T细胞、B细胞、朗格汉斯细胞、嗜酸性粒细胞、嗜中性粒细胞，和肥大细胞对48例患者同侧的NP组织和中鼻甲（MT）以及对照组的MT进行了成对的活检。用UniCAP（Pharmacia & Upjohn AB，Uppsala，Sweden）系统检测血清总IgE和常见局部过敏原特异性IgE。在鼻息肉患者的配对样本之间证明了细胞评分的强相关性。鼻息肉中的细胞类型主要表现为细胞浸润，CD 8 + T细胞、嗜酸性粒细胞和中性粒细胞评分显著升高，CD 4 +/CD 8 + T细胞中位数比值与对照组MT相比呈反比。结论：鼻息肉中粘膜炎症的免疫组化模式与配对的MT粘膜相似，提示鼻息肉的粘膜广泛受累。这表明除了手术去除NP之外，还需要对邻近鼻粘膜的变化进行抗炎治疗。除了在高加索人研究中公认的嗜酸性粒细胞炎症外，T细胞，尤其是CD 8 + T细胞的主要浸润可能是NPs发病机制的关键组成部分。
BACKGROUND: Nasal polyp (NP) is the most common mass lesion in the nose with unclear etiology and pathogenesis.OBJECTIVE: To investigate the association of allergy and type of cellular inflammation in Asian patients with NPs.METHODS: Immunohistochemical staining with a panel of antibodies For CD4+ and CD8+ T cells, B cells, Langerhans, cells, eosinophils, neutrophils, and Mast cells Were performed ill pairs of NP tissue and middle turbinate (MT) biopsies from the same side of 48 patients and MT of controls. Serum total IgE and specific to it panel of common local allergens were tested by the UniCAP (Pharmacia & Upjohn AB, Uppsala, Sweden) system.RESULTS: Atopy was found in 29.7% of NP patients. Strong correlations of cell scores were evidenced between the paired samples from nasal polyp patients. The cell patterns in nasal polyp mainly showed it combined cell infiltration with significantly higher CD8+ T cell, eosinophil and neutrophil scores, and an inverse median ratio of CD4+/CD8+ T cells as compared with the MT from controls.CONCLUSION: The similar immunohistochemical pattern of mucosal inflammation in NPs and the paired MT mucosa suggests a diffuse mucosal involvement. This indicates the necessity of anti-inflammatory treatment of changes in the adjacent nasal mucosa in addition to the Surgical removal of NPs. Besides the well-recognized eosinophilic inflammation in Caucasian Studies, predominant infiltration of T cells, especially CD8+ T cells, Could be it key component underlying the pathogeneses of NPs.