SURFACE-PROPERTY CHANGES FROM INTERACTIONS OF ALBUMIN WITH NATURAL LUNG SURFACTANT AND EXTRACTED LUNG LIPIDS
SURFACE-PROPERTY CHANGES FROM INTERACTIONS OF ALBUMIN WITH NATURAL LUNG SURFACTANT AND EXTRACTED LUNG LIPIDS
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DOI：
10.1016/0009-3084(85)90022-2
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发表时间：
1985-01-01
影响因子：
3.4
通讯作者：
FINKELSTEIN, JN
中科院分区：
文献类型：
--
作者：
HOLM, BA;NOTTER, RH;FINKELSTEIN, JN
这些实验表征了在生理温度、湿度和振荡气泡装置上的薄膜循环速率下，白蛋白对天然肺表面活性剂 (LS) 和提取的混合脂质成分 (CLL) 动态表面活性的影响。还报道了白蛋白对 CLL 和 LS 的表面压力-时间 (.pi.-t) 吸附等温线影响的测量。结果表明，37°C 时，浓度 (< 20 mg/ml) 的白蛋白将 LS 悬浮液 (0.4 mg 磷脂/ml) 的最小动态表面张力从 < 1 达因/厘米增加至 21 达因/厘米。低浓度 (2 mg/ml) 的白蛋白对提取的表面活性剂脂质 CLL 的动态表面活性具有类似的不利影响。此外，白蛋白还抑制 LS 和 CLL 的分离吸附能力；在血浆蛋白存在下，两种表面活性剂混合物（0.063 和 0.125 mg 磷脂/ml）的吸附速度较慢或达到较低的最终表面压力，而不是快速吸附到 45 达因/厘米的最大扩散压力。一个惊人的发现是，在高脂质浓度下，白蛋白对表面活性的抑制作用被减弱或消除。 LS 浓度 > 0.75 mg/ml，CLL 浓度 > 1.5 mg/ml，即使存在大量白蛋白 (100 mg/ml)，类似地，在足够高的磷脂浓度下，白蛋白对天然 LS 吸附的抑制也受到保护。这些结果表明，血浆渗出物中的白蛋白有可能严重损害。然而，如果存在磷脂的临界阈值，则有害影响将会减轻。
These experiments characterize the effects of albumin on the dynamic surface activity of natural lung surfactant (LS), and an extracted mixed lipid fraction (CLL), at physiologic temperature, humidity, and film cycling rate on an oscillating bubble apparatus. Measurements of albumin effects on the surface pressure-time (.pi.-t) adsorption isotherms of CLL and LS are also reported. Results show that albumin in concentrations (< 20 mg/ml increased the minimum dynamic surface tension of LS suspensions (0.4 mg phospholipid/ml) from < 1 dyne/cm to 21 dynes/cm at 37.degree. C. Albumin in low concentrations (2 mg/ml) had a similar detrimental effect on the dynamic surface activity of extracted surfactant lipids, CLL. In addition, albumin also inhibited the isolated adsorption facility of LS and CLL; instead of adsorbing rapidly to their maximum spreading pressures of 45 dynes/cm, both surfactant mixtures (at 0.063 and 0.125 mg phospholipid/ml) adsorbed more slowly or reached lower final surface pressures in the presence of plasma protein. A striking finding was that albumin inhibition of surface activity was moderated or abolished at high lipid concentrations. For example, minimum dynamic surface tension < 1 dyne/cm were reached on the oscillating bubble for natural LS at concentrations > 0.75 mg/ml and CLL at concentrations > 1.5 mg/ml, even in the presence of very large amounts of albumin (100 mg/ml). Similarly, LS and CLL adsorption facility was protected from albumin inhibition at sufficiently high phospholipid concentrations. Albumin inhibition of natural LS adsorption was also moderated by the presence of 1.4 mM Ca2+ ions. These results show that albumin in plasma transudates has the potential to seriously impair alveolar surfactant activity in vivo. However, the detrimental effect will be mitigated if a critical threshold of phospholipid is present.