Centrifugal partition chromatography in a biorefinery context: Optimisation and scale-up of monosaccharide fractionation from hydrolysed sugar beet pulp.
Centrifugal partition chromatography in a biorefinery context: Optimisation and scale-up of monosaccharide fractionation from hydrolysed sugar beet pulp.
复制标题
生物精炼厂中的离心分配色谱：水解甜菜浆中单糖分级的优化和放大。
DOI：
10.1016/j.chroma.2017.03.003
复制
发表时间：
2017
期刊：
影响因子：
--
通讯作者：
G. Lye
中科院分区：
文献类型：
--
作者：
David P. Ward;P. Hewitson;Max Cárdenas‐Fernández;Charlotte Hamley;A. Diaz‐Rodriguez;N. Douillet;Joseph P. Adams;D. Leak;S. Ignatova;G. Lye
从生物质中分离组分糖代表了可持续原料如甜菜浆的生物加工中的重要步骤。离心分配色谱法（CPC）在这里使用，作为一种替代多个树脂色谱步骤，从粗水解甜菜浆果胶组分单糖。在固定相中制备的样品的CPC分离使用乙醇：硫酸铵（300 g L−1）相系统（0.8：1.8 v：v）以上升模式进行。这使得能够在单个步骤中去除粗进料流杂质并将单糖分离成三个级分（l-鼠李糖、l-阿拉伯糖和d-半乳糖以及d-半乳糖醛酸）。通过增加样品进样体积，从柱体积的4%增加到16%，可将分离量提高三倍，在所有情况下均保持类似的分离性能。最终的半乳糖醛酸馏分的挤出增加了洗脱的溶质浓度，减少了24%的总分离时间，并消除了进一步柱再生的需要。通过使用多次堆叠进样验证了挤出后分离的重现性。从半制备型250 mL柱到制备型950 mL柱进行线性放大，放大比为3.8，适用于移动的流速和样品进样体积。在制备规模下实现了9.4 g L−1h− 1总溶解固体的生产量，其中组分单糖的生产量为1.9 g L−1h− 1。这些结果证明了CPC在生物精炼分离中用于杂质去除和目标分馏的潜力。
The isolation of component sugars from biomass represents an important step in the bioprocessing of sustainable feedstocks such as sugar beet pulp. Centrifugal partition chromatography (CPC) is used here, as an alternative to multiple resin chromatography steps, to fractionate component monosaccharides from crude hydrolysed sugar beet pulp pectin. CPC separation of samples, prepared in the stationary phase, was carried out using an ethanol: ammonium sulphate (300 g L−1) phase system (0.8:1.8 v:v) in ascending mode. This enabled removal of crude feedstream impurities and separation of monosaccharides into three fractions (l-rhamnose,l-arabinose andd-galactose, andd-galacturonic acid) in a single step. Throughput was improved three-fold by increasing sample injection volume, from 4 to 16% of column volume, with similar separation performance maintained in all cases. Extrusion of the final galacturonic acid fraction increased the eluted solute concentration, reduced the total separation time by 24% and removed the need for further column regeneration. Reproducibility of the separation after extrusion was validated by using multiple stacked injections. Scale-up was performed linearly from a semi-preparative 250 mL column to a preparative 950 mL column with a scale-up ratio of 3.8 applied to mobile phase flow rate and sample injection volume. Throughputs of 9.4 g L−1h−1of total dissolved solids were achieved at the preparative scale with a throughput of 1.9 g L−1h−1of component monosaccharides. These results demonstrate the potential of CPC for both impurity removal and target fractionation within biorefinery separations.
影响因子：
11.4
作者：
Hamley-Bennett, C.;Lye, G. J.;Leak, D. J.
通讯作者：
Leak, D. J.