Derivatization followed by reductive cleavage (DFRC method), a new method for lignin analysis: Protocol for analysis of DFRC monomers
Derivatization followed by reductive cleavage (DFRC method), a new method for lignin analysis: Protocol for analysis of DFRC monomers
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DOI：
10.1021/jf970258h
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发表时间：
1997-07-01
影响因子：
6.1
通讯作者：
Ralph, J
中科院分区：
文献类型：
--
作者：
Lu, FC;Ralph, J
本文介绍了一种选择性、高效地裂解木质素中芳基甘油-β-芳基（β-O-4）醚键的新方法。首字母缩略词“DFRC "涉及所涉及的反应，即衍生化和还原裂解。衍生化，伴随着细胞壁溶解，是用乙酰溴在乙酸中完成的;所产生的β-溴醚的还原裂解利用锌在酸性介质中。乙酰化后，通过GC定量降解单体（4-乙酰氧基肉桂基乙酸酯），提供与分析性硫代酸解类似的数据。
A new method far selective and efficient cleavage of arylglycerol-beta-aryl (beta-O-4) ether linkages in lignins is introduced. The acronym ''DFRC'' relates to the reactions involved, derivatization followed by reductive cleavage. Derivatization, accompanied by cell wall solubilization, is accomplished with acetyl bromide in acetic acid; reductive cleavage of resulting beta-bromo ethers utilizes zinc in an acidic medium. Following acetylation, degradation monomers (4-acetoxycinnamyl acetates) are quantified by GC, providing data analogous to those from analytical thioacidolysis.