CDNA SEQUENCES FOR PEA DISEASE RESISTANCE RESPONSE GENES
CDNA SEQUENCES FOR PEA DISEASE RESISTANCE RESPONSE GENES
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DOI：
10.1007/bf00017470
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发表时间：
1988-01-01
影响因子：
5.1
通讯作者：
HADWIGER, LA
中科院分区：
文献类型：
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作者：
FRISTENSKY, B;HOROVITZ, D;HADWIGER, LA
油菜I族抗病应答基因（pI49、pI176和pI206）的表达。阿拉斯加与番茄镰刀菌、丁香假单胞菌的耐药性相互作用密切相关。和真菌激发剂壳聚糖。将这些基因的cdna重新克隆到Bluescript KSm13+载体中并测序。pI49和pI176 cdna的最佳比对导致95%的总体同一性，表明这些基因可能是多基因家族的一部分。pI206的cDNA出现了5 '的截断。尽管Northern分析测得pI206的mRNA大约有950个核苷酸，但整个cDNA（不包括poly(A) tail）只有594 bp长。pI49和pI176的预测分子量分别为16728和16901 Da，而pI206的基因产物被认为至少为19360 Da。158个氨基酸的PI49序列与159个氨基酸的PI176序列仅相差5个氨基酸替换和1个插入/删除。
The expression of the Group I disease resistance response genes (pI49, pI176, and pI206) ofPisum sativumcv. Alaska is strongly associated with resistance interactions withFusarium solani, Pseudomonas syringaepv.pisi,and the fungal elicitor chitosan. The cDNAs for these genes were recloned into the Bluescript KSm13+ vector and sequenced. Optimal alignment of the pI49 and pI176 cDNAs resulted in an overall identity of 95%, suggesting that these genes may be part of a multigene family. The cDNA for pI206 appeared to be 5′-truncated. Although the mRNA for pI206 as measured by Northern analysis was approximately 950 nucleotides, the entire cDNA, excluding the poly(A) tail, was only 594 bp long. The predicted molecular weights of pI49 and pI176 are 16728 and 16901 Da, respectively, while the pI206 gene product is thought to be at least 19360 Da. The 158 amino acid PI49 sequence differed by only 5 amino acid substitutions and one insertion/deletion from the 159 amino acid PI176 sequence.