Nuclear pattern of CXCR4 expression is associated with a better overall survival in patients with gastric cancer.
Nuclear pattern of CXCR4 expression is associated with a better overall survival in patients with gastric cancer.
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CXCR4表达的核模式与胃癌患者的总体生存更好。
DOI：
10.1155/2014/808012
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发表时间：
2014
影响因子：
--
通讯作者：
Nikzaban M
中科院分区：
文献类型：
--
作者：
Nikkhoo B;Jalili A;Fakhari S;Sheikhesmaili F;Fathi F;Rooshani D;Hoseinpour Feizi MA;Nikzaban M
导论.已有研究表明，基质衍生因子-1（CXCL 12）及其受体CXCR 4在多种肿瘤的转移中起着至关重要的作用。类似地，已经清楚CXCR 4在胃癌的细胞表面上表达。然而，CXCR 4的核表达及其临床意义尚未研究。材料和方法。本研究采用RT-PCR、免疫组化和流式细胞术等技术检测了CXCR 4在胃腺癌患者胃组织和人胃癌细胞系AGS中的表达。结果RT-PCR结果显示CXCR 4在AGS细胞中有高表达。这通过IHC和FACS证实，因为在AGS细胞的细胞膜、细胞质和细胞核中检测到CXCR 4。此外，我们发现细胞质和细胞核CXCR 4在原发性胃癌中均强烈表达，并且CXCR 4的细胞质模式往往与比细胞核染色更短的总生存期相关。综上所述，我们首次提出了CXCR 4在胃癌组织中胞浆和胞核均表达的证据。然而，细胞质和细胞核CXCR 4的作用需要进一步阐明。
Introduction. Previous studies have shown that stromal-derived factor-1 (CXCL12) and its receptor, CXCR4, play a crucial role in metastasis of various tumors. Similarly, it has been cleared that CXCR4 is expressed on the cell surface of gastric cancers. However, nuclear expression of CXCR4 and its clinical importance have not been yet studied. Materials and Methods. Herein, we studied the expression of CXCR4 in gastric samples from patients with gastric adenocarcinoma as well as human gastric carcinoma cell line, AGS, by employing RT-PCR, immunohistochemistry, and flow cytometry techniques. Results. RT-PCR data showed that CXCR4 is highly expressed on AGS cells. This was confirmed by IHC and FACS as CXCR4 was detected on cell membrane, in cytoplasm, and in nucleus of AGS cells. Moreover, we found that both cytoplasmic and nuclear CXCR4 are strongly expressed in primary gastric cancer and the cytoplasmic pattern of CXCR4 tends to be associated with a shorter overall survival than nuclear staining. In conclusion, we present evidence for the first time that both cytoplasmic and nuclear expression of CXCR4 are detectable in gastric cancer tissues. However, the role of both cytoplasmic and nuclear CXCR4 needs to be further elucidated.