Genomic diversity in the Leishmania donovani complex
Genomic diversity in the Leishmania donovani complex
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DOI：
10.1017/s0031182099004710
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发表时间：
1999-09-01
期刊：
影响因子：
2.4
通讯作者：
Miles, MA
中科院分区：
文献类型：
--
作者：
Mauricio, IL;Howard, MK;Miles, MA
杜氏利什曼原虫复合体被认为由 3 个物种组成； L. donovani、L、infantum 和 L. chagasi，尽管这种分类受到了挑战。通过以下方式在不同水平上评估复合物内的基因型关系： 探针 Lmet9 的结合，对恰加斯利什曼原虫和旧世界利什曼原虫属具有特异性；组成型主要表面蛋白酶单基因 (mspC) 的部分测序和多态性 DNA 的随机扩增 (RAPD)。旧世界利什曼原虫属和杜氏利什曼原虫复合体具有单系起源。恰加斯利什曼原虫显然属于多诺瓦尼利什曼原虫复合体，但它与婴儿利什曼原虫无法区分，这表明恰加斯利什曼原虫在近代历史中被引入新大陆。婴儿利什曼原虫/L.恰加西被鉴定为杜氏乳杆菌复合体中的一个单系类群，但杜氏乳杆菌可能是并系群。 L. donovani 内部的多样性是巨大的，并且发现了关联的系统地理学模式。
The Leishmania donovani complex is considered to be composed of 3 species; L. donovani, L, infantum and L. chagasi, although this classification has been challenged. Genotypic relationships within the complex were evaluated at different levels by: binding of the probe Lmet9, specific for L. chagasi and Old World Leishmania spp.; partial sequencing of a constitutive major surface protease single gene (mspC) and random amplification of polymorphic DNA (RAPD). The Old World Leishmania spp, and the L. donovani complex have a monophyletic origin. Leishmania chagasi clearly belongs to the L, donovani complex but it is indistinguishable from L. infantum, which suggests introduction of L. chagasi into the New World in recent history. Leishmania infantum/L. chagasi was identified as a monophyletic group within the L. donovani complex but L. donovani may be paraphyletic. Diversity within L. donovani is substantial and phylogeographical patterns of association were found.