Microiodometric Determination of β-Lactamase Activity
Microiodometric Determination of β-Lactamase Activity
复制标题
微量碘量法测定β-内酰胺酶活性
DOI：
10.1128/aac.1.2.94
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发表时间：
1972
影响因子：
4.9
通讯作者：
K. Nordström
中科院分区：
文献类型：
--
作者：
R. Sykes;K. Nordström
β-内酰胺酶活性的产物（在青霉素的情况下为青霉噻唑酸）被碘化学计量氧化。因此，β-内酰胺酶活性可以通过蓝色淀粉-碘复合物的脱色来测量。由于脱色是一个缓慢的过程，脱色率得到了低估的速率，青霉素水解，直到15至20分钟后获得一个稳定的状态。如果反应混合物（3毫升）含有不超过0.001单位的酶，一个正确的测定β-内酰胺酶的活性得到了微量碘量法描述。该方法灵敏度高，并有一定的应用价值.
The product of β-lactamase activity (a penicilloic acid in the case of a penicillin) is stoichiometrically oxidized by iodine. Hence, the β-lactamase activity can be measured as decolorization of the blue starch-iodine complex. Since the decolorization is a slow process, the rate of decolorization gave an underestimate of the rate of penicillin hydrolysis until a steady state was obtained after 15 to 20 min. If the reaction mixture (3 ml) contained no more than 0.001 unit of enzyme, a correct determination of β-lactamase activity was obtained from the microiodometric method described. The method is sensitive and some applications are mentioned.