Chronic deep brain stimulation in the rat nucleus accumbens and its effect on morphine reinforcement
Chronic deep brain stimulation in the rat nucleus accumbens and its effect on morphine reinforcement
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DOI：
10.1111/j.1369-1600.2007.00088.x
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发表时间：
2008-03-01
影响因子：
3.4
通讯作者：
Wang, Chen-Guang
中科院分区：
文献类型：
--
作者：
Liu, Hong-Yu;Jin, Jie;Wang, Chen-Guang
为了探索治疗药物滥用的新方法，我们评估了慢性脑深部刺激（DBS）大鼠延髓核（NAc）对吗啡强化的影响，使用DBS装置和植入方法，我们开发。将32只体重240-260 g的成年大鼠分为DBS组（n = 10）、假DBS组（n = 12）和对照组（n = 10），DBS组给予手术、吗啡和DBS，假DBS组给予手术和吗啡，对照组给予生理盐水。将DBS电极立体定向植入单侧NAc核心，并连接到植入式脉冲发生器。然后，将它们固定在老鼠头骨上。一周后，各组大鼠腹腔注射吗啡，剂量递增（10-60 mg/kg），每日一次。DBS组给予130 Hz高频刺激（HFS），每日1次。采用900秒条件性位置偏爱（CPP）实验范式，观察电刺激对吗啡强化的影响。数据显示，7-10天后，DBS组的偏好评分显著低于假DBS组。结果提示，慢性高频刺激大鼠NAc可阻断吗啡诱导的位置偏爱，减弱吗啡的强化作用。
In order to explore a novel method for the treatment of drug abuse, we evaluated the effect of chronic deep brain stimulation (DBS) of the rat nucleus accumbens (NAc) on morphine reinforcement, using a DBS apparatus and an implant method we developed. Thirty-two adult rats weighing 240-260 g were divided into three groups, which included a DBS group (n = 10, administration of surgery, morphine and DBS), a sham DBS group (n = 12, administration of surgery and morphine) and a control group (n = 10, administration of physiological saline). The DBS electrode was stereotaxically implanted into the core of unilateral NAc and connected to an implantable pulse generator. Then, they were fixed to the rat skull. One week later, the rats in each group were intraperitoneally injected with morphine at an increasing dose (10-60 mg/kg) once daily. The rats in the DBS group were administered a 130-Hz high-frequency stimulation (HFS) once daily. A 900-second conditioned place preference (CPP) paradigm was used for determining the effect of electrical stimulation on morphine reinforcement in rats. The data showed that 7-10 days later, the preference score of the DBS group was significantly lower than that of the sham DBS group. The results suggest that chronic HFS of the rat NAc can block CPP induced by morphine and attenuate morphine reinforcement.