Characterization of P69E and P69F, two differentially regulated genes encoding new members of the subtilisin-like proteinase family from tomato plants
Characterization of P69E and P69F, two differentially regulated genes encoding new members of the subtilisin-like proteinase family from tomato plants
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DOI：
10.1104/pp.122.1.67
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发表时间：
2000-01-01
期刊：
影响因子：
7.4
通讯作者：
Vera, P
中科院分区：
文献类型：
--
作者：
Jordá, L;Conejero, V;Vera, P
枯草杆菌蛋白酶样蛋白质代表了一个古老的丝氨酸蛋白酶家族，在生物体中极为广泛存在。我们在这里报告了两个新的转录活性基因的结构和基因组组织，它们编码属于来自番茄（Lycopersicon esculentum）植物的枯草杆菌蛋白酶样蛋白酶 P69 家族的蛋白质。两个新成员P69E和P69F以簇的形式组织起来，并以串联的形式排列。对用启动子-β-葡萄糖醛酸酶融合体转化的转基因拟南芥植物的这两个基因的mRNA表达分析和研究表明，它们受到差异性调节，每个基因都显示出高度特异性的mRNA表达模式。 P69E mRNA 仅在根中表达，而 P69F mRNA 仅在水囊中表达。所有 P69 氨基酸序列、基因结构、表达谱和聚类组织的比较提出了 P69 基因家族进化的工作模型。
Subtilisin-like proteins represent an ancient family of serine proteases that are extremely widespread in living organisms. We report here the structure and genomic organization of two new transcriptionally active genes encoding proteins that belong to the P69 family of subtilisin-like proteases from tomato (Lycopersicon esculentum) plants. The two new members, P69E and P69F, are organized in a cluster and arranged in a tandem form. mRNA expression analysis and studies of transgenic Arabidopsis plants transformed with promoter-P-glucuronidase fusions for each of these two genes revealed that they are differentially regulated, with each showing a highly specific mRNA expression pattern. P69E mRNA is expressed only in roots, while P69F mRNA is expressed only in hydathodes. A comparison of all the P69 amino acid sequences, gene structure, expression profiles, and clustered organization suggests a working model for P69 gene family evolution.