Solute accumulation in the deep-sea bacterium Photobacterium profundum
Solute accumulation in the deep-sea bacterium Photobacterium profundum
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DOI：
10.1007/s00792-002-0288-1
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发表时间：
2002-12-01
期刊：
影响因子：
2.9
通讯作者：
Roberts, MF
中科院分区：
文献类型：
--
作者：
Martin, DD;Bartlett, DH;Roberts, MF
利用核磁共振技术研究了深海细菌深层发光杆菌菌株SS 9的细胞内溶质的特性和数量。P profundum菌株SS 9是一种中度嗜压菌，其在20-30 MPa下生长最佳，主要积累谷氨酸和甜菜碱，当在0.1 MPa下生长至早期稳定期时，具有较少量的丙氨酸、β-羟基丁酸（β-HB）和由β-HB单元组成的低聚物。当在最佳压力下生长时，细胞优先增加β-HB和β-HB寡聚体的细胞内浓度，而氨基酸库保持相对恒定。由于有机溶质随着介质中外部NaCl的增加而增加，因此它们起着渗透剂的作用。β-HB分子代表了一类新的渗透剂，称为“压渗剂”，其细胞水平对静水压力和渗透压作出反应。还考察了细胞生长阶段和温度等因素对溶质在这些细胞中分布的影响。
The identity and amounts of intracellular solutes in the deep-sea bacterium Photobacterium profundum strain SS9 were studied using nuclear magnetic resonance techniques. P profundum strain SS9, a moderate piezophile which grows optimally at 20-30 MPa primarily accumulated glutamate and betaine, with lesser amounts of alanine, beta-hydroxybutyrate (beta-HB) and oligomers composed of the beta-HB units when grown at 0.1 MPa to early stationary phase. When grown at the optimal pressure, the cells preferentially increased intracellular concentrations of beta-HB and beta-HB oligomers, while the amino acid pools remained relatively constant. Since the organic solutes increased with increasing external NaCl in the medium, they are functioning as osmolytes. The beta-HB molecules represent a novel class of osmolytes, termed "piezolytes," whose cellular levels responded to hydrostatic pressure as well as osmotic pressure. Factors such as cell growth stage and temperature were also examined for their effect on the solute distribution in these cells.