Genetic methods for analysis and manipulation of inversion mutations in bacteria.
Genetic methods for analysis and manipulation of inversion mutations in bacteria.
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用于分析和操作细菌反转突变的遗传方法。
DOI：
10.1093/genetics/105.3.517
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发表时间：
1983
期刊：
影响因子：
3.3
通讯作者：
Roth,JR
中科院分区：
文献类型：
--
作者：
Schmid,MB;Roth,JR
本文介绍了一些用于分离、鉴定和操纵鼠伤寒沙门氏菌大染色体倒位的遗传学方法。一个携带倒位的突变体被详细描述，并用于证明细菌倒位的许多独特的遗传特性。与预期相反，发现大的倒位突变可以通过广义转导修复。修复的结果从两个野生型转导片段同时引入到一个单一的受体细胞。两个转导片段和两个倒位断点之间的同源重组导致倒位片段再倒位。这导致该染色体片段的野生型取向的再生。类似的重组事件允许将大倒位突变引入野生型菌株;来自倒位菌株的两个转导片段引起重组事件，导致大染色体区段的倒位。描述和测试了通过广义转导映射大倒位突变的程度的遗传方法。该方法操作简单，并允许两个反演断点的良好分辨率。
A number of genetic methods for the isolation, characterization and manipulation of large chromosomal inversions inSalmonella typhimuriumare described. One inversion-carrying mutant is characterized in detail and used to demonstrate a number of unique genetic properties of bacterial inversions.—Contrary to expectation, it was found that large inversion mutations can be repaired by generalized transduction. The repair results from the simultaneous introduction of two wild-type transduced fragments into a single recipient cell. Homologous recombination between the two transduced fragments and the two inversion breakpoints causes the inverted segment to be reinverted. This results in regeneration of the wild-type orientation of this chromosome segment. Similar recombination events allow a large inversion mutation to be introduced into a wild-type strain; two transduced fragments from an inversion strain cause recombination events resulting in inversion of a large chromosome segment.—Genetic methods for mapping the extent of a large inversion mutation by generalized transduction are described and tested. The methods are operationally simple and allow good resolution of the two inversion breakpoints.