Immunolocalization of LeAGP-1, a modular arabinogalactan-protein, reveals its developmentally regulated expression in tomato
Immunolocalization of LeAGP-1, a modular arabinogalactan-protein, reveals its developmentally regulated expression in tomato
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DOI：
10.1007/s004250050691
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发表时间：
2000-05-01
期刊：
影响因子：
4.3
通讯作者：
Showalter, AM
中科院分区：
文献类型：
--
作者：
Gao, MG;Showalter, AM
阿拉伯半乳糖蛋白（AGPs）是高度糖基化的细胞表面蛋白，被认为在植物生长发育中起作用。以番茄为研究对象，研究了一种新型的主要AGP-1在不同器官组织中的发育调控表达。简历。UC82B)植物具有抗肽抗体（即PAP抗体），特异性针对LeAGP-1核心蛋白的赖氨酸丰富亚结构域。在番茄细胞分化过程中，LeAGP-1与特定类型细胞的细胞壁增厚和木质化有关。具体来说，在根和茎成熟的木质部和次生木质部管状部的次生壁增厚以及韧皮部筛状部的细胞壁增厚中检测到LeACP-1。然而，LeAGP-1也存在于幼苗根的薄壁皮质薄壁细胞和幼茎的厚壁厚壁厚壁细胞中，这两种细胞都没有木质化。基于这些观察到的模式，讨论了legp -1在植物生长发育中的可能作用。
Arabinogalactan-proteins (AGPs) are highly glycosylated cell surface proteins that are thought to function in plant growth and development. The developmentally regulated expression of LeAGP-1, a novel and major AGP in tomato, was examined in different organs and tissues of tomato (Lycopersicon esculentum Mill. cv. UC82B) plants with an anti-peptide antibody (i.e. the PAP antibody) directed specifically against the lysine-rich subdomain of the LeAGP-1 core protein. During cell differentiation in tomato plants, LeAGP-1 was associated with cell wall thickening and lignification of particular cell types. Specifically, LeACP-1 was detected in secondary wall thickenings of maturing metaxylem and secondary xylem tracheary elements in roots and stems, and in thickened cell walls of phloem sieve elements. However, LeAGP-1 was also present in thin-walled, cortical parenchyma cells of seedling roots as well as thick-walled collenchyma cells in young stems, both of which are not lignified. Based on these observed patterns, possible roles for LeAGP-1 in plant growth and development are discussed.