Effect of vasoactive intestinal peptide on ion transport across dog tracheal epithelium.
Effect of vasoactive intestinal peptide on ion transport across dog tracheal epithelium.
复制标题
血管活性肠肽对狗气管上皮离子转运的影响。
DOI：
10.1152/jappl.1983.55.6.1844
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Barnes,PJ
中科院分区：
文献类型：
--
作者：
Nathanson,I;Widdicombe,JH;Barnes,PJ
在短路条件下，血管活性肠肽（VIP）不改变Na+的净运动，但选择性地刺激高亲和力（Km相当于10（-8）M）的犬气管上皮细胞的净Cl-分泌。Cl-分泌的增加与短路电流（Isc）的增加没有区别。然而，Cl-分泌的刺激不是最大的，因为在VIP处理的组织中加入异丙肾上腺素（10（-6）M）进一步增加了Isc 54%。阿托品、酚妥拉明、普萘洛尔（10（-5）或10（-6）M）或河豚毒素（10（-6）M）的组合不能阻断外源性VIP的作用。在开路条件下，VIP引起Cl-和Na+的净分泌增加，但变化未达到统计学意义。我们得出结论，VIP直接作用于上皮细胞表面的受体，刺激活性Cl-分泌。丰富的VIP神经在粘膜下层表明，VIP可能是重要的，在调节流体运动通过上皮。
Under short-circuit conditions, vasoactive intestinal peptide (VIP) did not alter net Na+ movement but selectively stimulated net Cl- secretion across dog tracheal epithelium with a high affinity (Km congruent to 10(-8) M). The increase in Cl- secretion was not different from the rise in short-circuit current (Isc). However, stimulation of Cl- secretion was not maximal, because the addition of isoproterenol (10(-6) M) to VIP-treated tissues further increased the Isc by 54%. The effect of exogenous VIP was not blocked by a combination of atropine, phentolamine, propranolol (10(-5) or 10(-6) M), or tetrodotoxin (10(-6) M). Under open-circuit conditions, VIP caused an increase in the net secretion of Cl- and Na+, but the changes did not reach statistical significance. We conclude that VIP acts directly on receptors on the surface of epithelial cells to stimulate active Cl- secretion. The abundance of VIP nerves in the submucosa suggests that VIP may be important in regulation of fluid movement across the epithelium.