Knock-down of a RING finger gene confers cold tolerance
Knock-down of a RING finger gene confers cold tolerance
复制标题
敲低RING指基因赋予耐寒性
DOI：
10.1080/21655979.2015.1131368
复制
发表时间：
2016-01-01
期刊：
影响因子：
4.9
通讯作者：
Huang, Ji
中科院分区：
文献类型：
--
作者：
Fang, Huimin;Meng, Qingling;Huang, Ji
摘要植物特异性RING结构域指蛋白在植物发育和逆境应答中起着重要作用。我们最近从水稻中鉴定了一个胁迫诱导的基因OsSRFP 1（Oryza sativa Stress-related RING Finger Protein 1），并对其功能进行了鉴定。OsSRFP 1基因敲除植株的抗寒性增强与较高的游离脯氨酸含量和抗氧化酶活性有关。体外泛素化实验表明OsSRFP 1具有E3泛素连接酶活性。OsSRFP 1的一些预测的相互作用伙伴可能是OsSRFP 1介导的蛋白质降解的底物。有趣的是，OsSRFP 1具有反式激活活性，提示OsSRFP 1在逆境应答中具有翻译后和转录调控的双重作用。
Abstract The plant-specific RING-domain finger proteins play important roles in plant development and stress responses. We recently identified and functionally characterized a stress-induced gene OsSRFP1 (Oryza sativa Stress-related RING Finger Protein 1) from rice.1 We showed evidences of the biotechnological potential of the suppression of OsSRFP1 expression in conferring cold tolerance. The increased cold tolerance of OsSRFP1 knock-down plants was associated with higher amounts of free proline and activities of antioxidant enzymes. In vitro ubiquitination assays showed that OsSRFP1 possessed E3 ubiquitin ligase activity. Some predicted interacting partners of OsSRFP1 might be the substrates for OsSRFP1-mediated protein degradation. Interestingly, OsSRFP1 had trans-activation activity, suggesting the dual roles of OsSRFP1 in post-translational and transcriptional regulations in stress responses.