T-CELLS OR ACTIVE EPSTEIN-BARR-VIRUS INFECTION IN THE DEVELOPMENT OF LYMPHOPROLIFERATIVE DISEASE IN HUMAN B-CELL-INJECTED SEVERE COMBINED IMMUNODEFICIENT MICE
T-CELLS OR ACTIVE EPSTEIN-BARR-VIRUS INFECTION IN THE DEVELOPMENT OF LYMPHOPROLIFERATIVE DISEASE IN HUMAN B-CELL-INJECTED SEVERE COMBINED IMMUNODEFICIENT MICE
复制标题
DOI：
10.1007/bf02303813
复制
发表时间：
1994-09-01
影响因子：
3.7
通讯作者：
LYERLY, HK
中科院分区：
文献类型：
--
作者：
COLES, RE;BOYLE, TJ;LYERLY, HK
Background: Severe combined immunodeficient (SCID) mice eve op Epstein-Barr virus (EBV) containing human lymphoproliferative disease (LPD) tumors when reconstituted with human peripheral blood leukocytes (PBLs) from EBV-seropositive donors, but LPD tumors do not develop in the presence of immunosuppressive agents, such as cyclosporine A or corticosteroids.Methods: Therefore, LPD development in SCID mice was used as a model to explore the relationship among B cells, T cells, and EBV in vivo. SCID mice were engrafted with PBLs isolated by leukapheresis from a single EBV-seropositive donor. Purified populations of CD3+ lymphocytes (T cells) or CD19+ lymphocytes (B cells) were isolated and engrafted into SCID mice.Results: SCID mice engrafted with purified CD3+ lymphocytes (T cells) or CD19+ lymphocytes (B cells) did not develop LPD. In contrast, mice engrafted with purified B cells developed LPD if they were co-engrafted with purified T cells or if they were inoculated with infectious EBV.Conclusions: This study confirms the requirement of T cells or active EBV infection in the development of LPD in animals engrafted with B cells latently infected with EBV. A greater understanding of the cellular and viral interactions leading to transformation and malignancy may allow the development of specific interventional therapies for malignancies in the immunosuppressed host.
