Theoretical description of the polymerase chain reaction
Theoretical description of the polymerase chain reaction
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DOI：
10.1006/jtbi.1997.0473
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发表时间：
1997-10-07
影响因子：
2
通讯作者：
Mendoza, C
中科院分区：
文献类型：
--
作者：
Schnell, S;Mendoza, C
考虑到最近从酶学的考虑推导出的聚合酶链反应（PCR）效率的表达式，并利用基于质量作用定律的连续模型，导出了封闭形式的解，可以完整地描述标准和定量竞争性PCR方法。由此产生的行为与先前的、经验的、离散的方法的行为是合理一致的；但是，由于在循环期间效率恒定的弱假设，后者被证明高估了放大产量多达30%。目前的形式将有助于实施准确的拟合程序的实验数据，以管理量化。(C) 1997学术出版社有限公司
Taking into account expressions for the efficiency of the polymerase chain reaction (PCR) recently deduced from enzymological considerations, and making use of a continuous model based on the law of mass action, closed form solutions are derived that enable a complete description of the standard and quantitative competitive PCR methods. The resulting behaviour is in reasonable agreement with that from a previous, empirical, discrete approach; but the latter is nonetheless shown to overestimate the amplification yield by as much as 30% due to the weak assumption of a constant efficiency during the cycle duration. The present formalism will facilitate the implementation of accurate fitting procedures of experimental data to manage quantification. (C) 1997 Academic Press Limited.