Distinguishing the cyanobacterial neurotoxin β-N-methylamino-L-alanine (BMAA) from other diamino acids
Distinguishing the cyanobacterial neurotoxin β-N-methylamino-L-alanine (BMAA) from other diamino acids
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DOI：
10.1016/j.toxicon.2011.02.005
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发表时间：
2011-04-01
期刊：
影响因子：
2.8
通讯作者：
Cox, P. A.
中科院分区：
文献类型：
--
作者：
Banack, S. A.;Metcalf, J. S.;Cox, P. A.
β-N-甲基氨基-γ-丙氨酸（BMAA）是由不同的蓝藻类群产生的，并且已经被许多研究者在蓝藻水华、培养物和收集物中发现。虽然BMAA是区别于蛋白质氨基酸和它的异构体2，4-DAB使用标准色谱和质谱技术常规用于分析氨基酸，我们研究了BMAA是否可以可靠地区分其他二氨基酸，特别是2，6-二氨基庚二酸已被分离出的细胞壁的许多细菌物种。我们使用HPLC-FD、UHPLC-UV、UHPLC-MS和三重四极杆串联质谱（UHPLC-MS/MS）将BMAA与二氨基酸2，6-二氨基庚二酸、N-2（氨基）乙基甘氨酸、赖氨酸、鸟氨酸、2，4-二氨基琥珀酸、高胱氨酸、胱氨酸、色氨酸以及其他氨基酸（包括天冬酰胺、谷氨酰胺和甲硫氨酸甲基锍）区分开来。(C)2011爱思唯尔有限公司保留所有权利。
beta-N-methylamino-r-alanine (BMAA) is produced by diverse taxa of cyanobacteria, and has been detected by many investigators who have searched for it in cyanobacterial blooms, cultures and collections. Although BMAA is distinguishable from proteinogenic amino acids and its isomer 2,4-DAB using standard chromatographic and mass spectroscopy techniques routinely used for the analysis of amino acids, we studied whether BMAA could be reliably distinguished from other diamino acids, particularly 2,6-diaminopimelic acid which has been isolated from the cell walls of many bacterial species. We used HPLC-FD, UHPLC-UV, UHPLC-MS, and triple quadrupole tandem mass spectrometry (UHPLC-MS/MS) to differentiate BMAA from the diamino acids 2,6-diaminopimelic acid, N-2(amino)ethylglycine, lysine, ornithine, 2,4-diaminosuccinic acid, homocystine, cystine, tryptophan, as well as other amino acids including asparagine, glutamine, and methionine methylsulfonium. (C) 2011 Elsevier Ltd. All rights reserved.