Development of phytoplasma-induced flower symptoms in Arabidopsis Thaliana
Development of phytoplasma-induced flower symptoms in Arabidopsis Thaliana
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DOI：
10.1016/j.pmpp.2011.09.001
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发表时间：
2011-12-01
影响因子：
2.7
通讯作者：
Firrao, Giuseppe
中科院分区：
文献类型：
--
作者：
Cettul, Emanuele;Firrao, Giuseppe
通过建立拟南芥与意大利三叶草叶根（ICPh）植物原体感染的有效方案，建立了植物与植物原体相互作用过程中导致花症状的分子事件研究的可靠模型。不同生态型拟南芥的ICPh植物原体感染导致了广泛的症状模式。选择Col-0生态型是基于可重复的时间过程和花发育变化的严重程度。用ICPh植原体在短时间内接种萌发后30 d的Col-0，产生的花症状越来越严重，从心皮修饰到绿化、叶状、确定性丧失和器官发育模式丧失。基因表达分析显示，与开花相关的同型基因和赤霉素代谢相关的基因明显下调。在植物血浆感染的野生型植物和健康突变体中发现了共同的形态特征，这些突变体的基因功能受损，在受感染的植物中被发现下调。形态学变化分析和实时荧光定量PCR结果表明，SEPALLATA基因在花发育和激素控制中发挥了核心作用。(C) 2011 Elsevier Ltd.版权所有。
A reliable model for the study of the molecular events leading to floral symptoms during plant-phytoplasma interaction was established by developing an efficient protocol for the infection of Arabidopsis thaliana with the Italian Clover Phyllody (ICPh) phytoplasma. ICPh phytoplasma infection of different A. thaliana ecotypes resulted in a wide range of symptom patterns. Ecotype Col-0 was selected for the repeatable time course and the severity of flower developmental alterations. By inoculating Col-0 at 30 days after germination with ICPh phytoplasma in short day, flowers were produced with increasingly severe symptoms, from carpels modification to virescence, phyllody, loss of determinacy and loss of organ developmental pattern. Gene expression analysis showed strong down regulation of some homeotic genes involved in flowering and genes involved in the gibberellin metabolism. Common morphological features were detected in phyto-plasma-infected wild type plants and healthy mutants impaired in the gene functions that were found to be down-regulated in the infected plants. Evidence from the analysis of morphological alterations and from the results of quantitative real time PCR pointed to a central role of the deregulation of SEPALLATA genes, that are involved in flower development and hormonal control. (C) 2011 Elsevier Ltd. All rights reserved.