Protein crystal growth in space, past and future
Protein crystal growth in space, past and future
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DOI：
10.1016/s0022-0248(01)02334-x
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发表时间：
2002-04-01
影响因子：
1.8
通讯作者：
Weise, L
中科院分区：
文献类型：
--
作者：
DeLucas, LJ;Moore, KM;Weise, L
伯明翰阿拉巴马大学生物物理科学与工程中心 (CBSE) 已在超过 39 次美国航天飞机任务中进行了蛋白质晶体生长实验。这些实验的结果清楚地表明，微重力环境是有益的，因为许多结晶的蛋白质比地球上生长的蛋白质更大、质量更高。晶体质量的改善是通过分析极限衍射分辨率、单个峰镶嵌度和电子密度图来判断的。现在有许多蛋白质晶体的分辨率提高了 0.5-1.5 埃。镶嵌性研究表明，微重力生长晶体的峰宽急剧减小。这些微重力结果加上来自其他各种研究人员的数据刺激了各个航天机构支持大分子晶体生长过程的基础研究。 CBSE 投入了大量精力开发动态控制晶体生长系统，使科学家能够优化地球或太空的结晶参数。这些系统能够监测和控制成核和成核后生长阶段的方法。从而显着改善晶体尺寸和X射线衍射特性。 CBSE 目前正在为国际空间站设计一个完整的晶体学实验室，包括： 晶体生长架。支持多种结晶硬件系统；用于晶体表征或完整 X 射线数据集收集的 X 射线衍射架：以及机器人控制的晶体采集/冷冻保存系统，可以通过远程机器人和/或机器人程序以最少的工作人员时间进行操作。 X 射线系统的关键要素包括独特的 X 射线聚焦技术和轻量化技术。低功率源。 X 射线检测系统基于石油商用 CCD 技术。本文将描述为国际空间站设想的 X 射线设施。 (C) 2002 Elsevier Science B.V. 保留所有权利。
The Center for Biophysical Sciences and Engineering (CBSE) at the University of Alabama at Birmingham has performed protein crystal growth experiments on more than 39 US space Shuttle missions. Results from these experiments have clearly demonstrated that the microgravity environment is beneficial in that a number of proteins crystallized were larger and of higher quality than their Earth-grown Counterparts. Improvement in crystal quality is judged by analysis of ultimate diffraction resolution, individual peak mosaicity, and electron density maps. There are now a number of protein crystals that exhibited resolution improvements of 0.5-1.5 Angstrom. Mosaicity Studies revealed dramatic decreases in peak widths for the microgravity-grown crystals. These microgravity results plus data from a variety of other investigators have stimulated Various space agencies to Support fundamental studies in macromolecular crystal growth processes. The CBSE has devoted substantial effort toward the development of dynamically controlled crystal growth systems which allow scientists to optimize crystallization parameters on Earth or in space. These systems enable monitoring and control of the approach to nucleation and post-nucleation growth phases. thereby dramatically improving the crystal size and X-ray diffraction characteristics. The CBSE is Currently designing a complete crystallographic laboratory for the international Space Station including: a crystal growth rack. which Will Support a variety of crystallization hardware systems; an X-ray diffraction rack for crystal characterization or a complete X-ray data set collection: and robotically controlled crystal harvesting/cryopreservation systems that can be operated with minimal crew time via telerobotic and/or robotic procedures. Key elements of the X-ray system include unique X-ray focusing technology combined with a lightweight. low-power Source. The X-ray detection system is based oil commercial CCD-based technology. This paper will describe the X-ray facility envisioned for the International Space Station. (C) 2002 Elsevier Science B.V. All rights reserved.