Pharmacological characterization of micropatterned cardiac myocytes
Pharmacological characterization of micropatterned cardiac myocytes
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DOI：
10.1016/s0142-9612(03)00275-8
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发表时间：
2003-10-01
期刊：
影响因子：
14
通讯作者：
Matsue, T
中科院分区：
文献类型：
--
作者：
Kaji, H;Takii, Y;Matsue, T
通过微接触印刷制备单细胞水平上的心肌细胞图案，并使用共焦荧光 Ca2+ 成像研究对化学刺激的反应。发现图案化的虹膜细胞通过形成间隙连接来缀合。已证实图案化的肌细胞间隙连接通讯被 1-辛醇可逆地抑制，但被咖啡因激活。还尝试使用显微注射系统用化学物质进行局部刺激，以形成由单细胞排列形成的肌细胞图案。本研究的目的是开发基于细胞网络的生物测定系统。 (C) 2003 Elsevier Science Ltd. 保留所有权利。
The cardiac myocytes patterned on a single cell level were prepared by microcontact printing, and the response to chemical stimuli was studied using confocal fluorescent Ca2+ imaging. The patterned iriyocytes were found to conjugate by forming gap junction. It was confirmed for the patterned myocytes that gap junction communication was reversibly inhibited by 1-octanol, but activated by caffeine. Localized stimulation with chemicals was also attempted using a microinjection system for the myocyte patterns formed by single cell alignment. This research was carried out with the objective of developing a bioassay system based on a cellular network. (C) 2003 Elsevier Science Ltd. All rights reserved.