TRANSFECTION OF PC12 CELLS WITH THE HUMAN GAP-43 GENE - EFFECTS ON NEURITE OUTGROWTH AND REGENERATION
TRANSFECTION OF PC12 CELLS WITH THE HUMAN GAP-43 GENE - EFFECTS ON NEURITE OUTGROWTH AND REGENERATION
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DOI：
10.1016/0169-328x(90)90071-k
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发表时间：
1990-01-01
期刊：
影响因子：
--
通讯作者：
NEVE, RL
中科院分区：
文献类型：
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作者：
YANKNER, BA;BENOWITZ, LI;NEVE, RL
神经元生长相关蛋白 GAP-43 在轴突生长和再生过程中高水平表达。在本报告中，我们描述了在莫洛尼鼠白血病病毒长末端重复序列 (MoMuLV LTR) 的控制下，用人 GAP-43 cDNA 转染神经生长因子 (NGF) 反应性嗜铬细胞瘤细胞系 PC12。分离出两个 PC12 亚克隆，它们从转染的 cDNA 中组成型表达 GAP-43，并显示出对 NGF 的反应性增加。在两个转染的 PC12 亚克隆中，表达最多人 GAP-43 RNA 的亚克隆表现出加速的初始神经突生长反应以及对 NGF 的敏感性增加 10 倍。在两个转染的亚克隆中，神经突再生均显着增强，并且与未处理的 PC12 细胞相比，在不添加 NGF 的情况下，神经突再生可能会短暂发生。这些结果表明 GAP-43 可能增强 NGF 对神经突起始和再生的作用。
The neuronal growth associated protein GAP-43 is expressed at high levels during axonal growth and regeneration. In this report, we describe the transfection of the nerve growth factor (NGF)-responsive pheochromocytoma cell line PC12 with the human GAP-43 cDNA under the control of the Moloney murine leukemia virus long terminal repeat (MoMuLV LTR). Two PC12 subclones were isolated that constitutively expressed GAP-43 from the transfected cDNA and showed increased responsiveness to NGF. Of the two transfected PC12 subclones, the subclone expressing the most human GAP-43 RNA showed an accelerated initial neurite outgrowth response and a 10-fold increased sensitivity to NGF. Neurite regeneration was significantly enhanced in both transfected subclones and, in contrast to untreated PC12 cells, could occur transiently in the absence of added NGF. These results suggest that GAP-43 may potentiate the action of NGF on neurite initiation and regeneration.