Poly(vinyl chloride)-coated sol-gels for studying the effects of nitric oxide release on bacterial adhesion
Poly(vinyl chloride)-coated sol-gels for studying the effects of nitric oxide release on bacterial adhesion
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DOI：
10.1021/bm049727w
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发表时间：
2004-09-01
影响因子：
6.2
通讯作者：
Schoenfisch, MH
中科院分区：
文献类型：
--
作者：
Nablo, BJ;Schoenfisch, MH
一氧化氮（NO）释放溶胶-凝胶材料涂有聚氯乙烯（PVC）膜表现出增加的稳定性，在环境和生理温度。然而，聚合物外涂层在释放的最初一周内将NO通量降低了5-35%。7天后，未改性和PVC涂覆的溶胶-凝胶之间的NO通量的变化可以忽略不计。PVC聚合物层提供受控的表面化学，用于系统研究NO释放对细菌粘附的影响。作为一个例子，铜绿假单胞菌和奇异变形杆菌粘附在PVC涂层的NO释放溶胶-凝胶进行了研究。当NO流量高达20 pmol cm（-2）s（-1）时，观察到铜绿假单胞菌粘附减少与NO直接相关。虽然在NO释放的存在下减少了50%，但奇异变形杆菌粘附似乎与NO释放的通量无关。PVC包覆的NO释放溶胶-凝胶可用于研究局部NO释放对其他生物和化学系统的影响。
Nitric oxide (NO) releasing sol-gel materials coated with poly(vinyl chloride) (PVC) films exhibit increased stability at ambient and physiological temperatures. The polymer overcoat, however, reduces the NO fluxes by 5-35% over the initial week of release. The variation in NO fluxes between unmodified and PVC-coated sol-gels is negligible after 7 days. The PVC polymeric layer provides controlled surface chemistry for systematic studies of the effects of NO release on bacterial adhesion. As an example, the adhesion of Pseudomonas aeruginosa and Proteus mirabilis at PVC-coated NO-releasing sol-gels is investigated. A direct NO dependence on the reduction of P. aeruginosa adhesion is observed for NO fluxes up to 20 pmol cm(-2) s(-1). Although decreased by 50% in the presence of NO release, P. mirabilis adhesion does not appear to correlate to the flux of NO release. PVC-coated NO-releasing sol-gels may prove useful for studying the effects of localized NO release on other biological and chemical systems.