[The level of matrix metalloproteinases and type IV collagen in the gingival mucosa under different clinical forms of periodontitis in pre-and pubertal periods and their prognostic value].
[The level of matrix metalloproteinases and type IV collagen in the gingival mucosa under different clinical forms of periodontitis in pre-and pubertal periods and their prognostic value].
复制标题
青春期前及青春期不同临床形式牙周炎牙龈黏膜基质金属蛋白酶和IV型胶原水平及其预后价值
作者：
Z. Tsagareli;T. Shishniashvili;L. Gogiashvili;T. Kvachadze;N. Khimshiashvili
本研究的目的是分析学龄青少年牙周病的患病率和严重程度，并探讨牙周组织的变化程度与青春期前和青春期内激素水平的关系。观察总数- 618名第比利斯9至17岁的学童。队列组（n=50）按年龄分为：1）9-13岁（n=26），2）14-17岁（n=24）。形态学和免疫组织化学研究的材料是根据正畸证词在拔牙时获得的牙周组织样品。用苏木精和伊红对牙龈粘膜的样品进行染色，在相同的样品中，使用一抗MMP-1和MMP-2（“Novocastra”，UK）通过免疫组织化学测定基质金属蛋白酶的表达和活性。免疫组化反应结果采用半定量方法进行评分。为了检测基底膜中的IV型胶原，将样品通过“直接红-80”（“Sigma”）染色。发现基质金属蛋白酶表达的增加和基底膜IV型胶原的变性具有年龄依赖性，特别是MMP-1和MMP-2在14-17岁（青春期）的表达高于9-13岁组。研究还证明了基质金属蛋白酶MMP-1，2活性的临床意义，表明细胞外物质和IV型胶原的解离，在评估青春期前和青春期牙周复合体病理的严重程度和预后。
The purpose of the study - the analysis of the prevalence and gravity of periodontal diseases among school adolescents and detection the relationship between the degree of periodontal tissues changes and the hormonal status of the prepubertal and pubertal periods. The total number of observations - 618 Tbilisi schoolchildren at the age of 9 to 17 years. Cohort group (n=50) was divided by age: 1) 9-13 years (n=26), 2) 14-17 years (n=24). The material for morphological and immunohistochemical study were the samples of periodontal tissues, received during the tooth extraction according the orthodontic testimony. The samples of the gingival mucosa was stained by hematoxylin and eosin, in the same samples the Matrix Metalloproteinases expression and activity were determined by immunohistochemistry using a primary antibody MMP-1 and MMP-2 ("Novocastra", UK). The results of immunohistochemical reactions were estimated in scores by semiquantitative method. For the detection of type IV collagen in basement membranes the samples were stained by the "Direct Red-80" ("Sigma"). It was found that the increase of Matrix Metalloproteinases expression and the type IV collagen degeneration in basement membrane depend on the age, particularly the expression of MMP-1 and MMP-2 in the period of 14-17 years (puberty) is upwards than in the group of 9-13 years. Studies have also demonstrated the clinical significance of Matrix Metalloproteinases ММР-1, 2 activity indicating the dissociation of the extracellular substance and type IV collagen in the assessment of severity and prognosis of periodontal complex pathology in pre-and pubertal periods.