An in vitro Assay of Erythropoietin.∗
An in vitro Assay of Erythropoietin.∗
复制标题
促红细胞生成素的体外测定。
DOI：
10.3181/00379727-125-32094
复制
发表时间：
1967
影响因子：
--
通讯作者：
H. P. Ward
中科院分区：
文献类型：
--
作者：
H. P. Ward
促红细胞生成素刺激Fe59与血红素结合的能力已得到证实。设计了一种体外骨髓培养测定促红细胞生成素的方法。灵敏度下限为0.05单位/ml，呈对数剂量响应关系。促红细胞生成素抗体、胰蛋白酶和放线菌素D阻断了体外促红细胞生成素的作用。
Summary The ability of erythropoietin to stimulate Fe59 incorporation into heme was confirmed. A method of bone marrow culture was designed in order to assay erythropoietin in vitro. A log-dose response relationship was demonstrated with a lower limit of sensitivity of 0.05 units/ml. The effect of erythropoietin in vitro was blocked by erythropoietin antibody, trypsin, and actinomycin D.