Detection of pathogen Escherichia coli O157:H7 using self-excited PZT-glass microcantilevers
Detection of pathogen Escherichia coli O157:H7 using self-excited PZT-glass microcantilevers
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DOI：
10.1016/j.bios.2004.11.009
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发表时间：
2005-09-15
影响因子：
12.6
通讯作者：
Mutharasan, R
中科院分区：
文献类型：
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作者：
Campbell, GA;Mutharasan, R
制备了长5和3 mm、宽1.8和2.0 mm的PZT-玻璃复合自激悬臂梁，并在空气中和液体浸没条件下测定了它们的谐振特性。在空气中，本文所用的两个悬臂梁在65.8和63.4kHz处发生共振。用单克隆抗体（MAb）对大肠杆菌（Escherichia coli，E. coli）O 157：H7固定在悬臂玻璃尖端，然后暴露于浓度范围为7 × 102至7 × 107个细菌/mL的病原体。共振的第二种模式下降，由于病原体附着在根据建议的动力学模型。发现特异性附着速率常数为3 x 10（-9）至5 x 10（-9）min（-1）（细胞/mL）（-1）。暴露于含有致病性和非致病性菌株的混合人群表明，抗体固定的悬臂梁是高度选择性的，从而证明其用于检测水传播的病原体的有用性。(c)2004 Elsevier B. V.保留所有权利。
Composite self-excited PZT-glass cantilevers (5 and 3 mm in length, 1.8 and 2.0 mm wide) were fabricated and their resonance characteristics were determined in air and at I turn liquid immersion. In air, resonance occurred at 65.8 and 63.4 kHz for the two cantilevers used in this paper. Monoclonal antibody (MAb) specific to the pathogen Escherichia coli (E. coli) O157:H7 was immobilized at the cantilever glass tip, and then exposed to pathogen in the concentration range of 7 x 102 to 7 x 10(7) bacteria/mL. Resonance of the second mode decreased due to pathogen attachment in accordance with a proposed kinetic model. The specific attachment rate constant was found to be 3 x 10(-9) to 5 x 10(-9) min(-1) (cell/mL)(-1). Exposure to a mixed population containing both a pathogenic and non-pathogenic strain showed that the antibody-immobilized cantilever is highly selective, thus demonstrating its usefulness for detecting water-borne pathogens. (c) 2004 Elsevier B.V. All rights reserved.