Macrophage-lymphocyte interaction in the antigen-induced blastogenic response of human peripheral blood leukocytes.
Macrophage-lymphocyte interaction in the antigen-induced blastogenic response of human peripheral blood leukocytes.
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人外周血白细胞抗原诱导的胚芽生成反应中巨噬细胞-淋巴细胞的相互作用。
DOI：
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发表时间：
1968
影响因子：
4.4
通讯作者：
Jules E. Harris
中科院分区：
文献类型：
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作者：
Evan M. Hersh;Jules E. Harris
在体外，与未分离的白细胞悬液相比，玻璃珠柱纯化的淋巴细胞悬液降低了对抗原的原始细胞反应。将这些纯化的淋巴细胞培养在巨噬细胞单层上，通常可以恢复细胞对抗原的反应。对植物血凝素(PHA)的反应不受柱纯化的影响。所用抗原为链溶素O(SLO)、链激酶型链多糖酶(SK-SD)、纯化蛋白衍生物(PPD)和天花疫苗(POX)。未分离的白细胞、分离的淋巴细胞和分离的淋巴细胞对PHA的反应率分别为76%、74%和74%，对SLO的反应率分别为18%、0.0%和10.0%，对SK-SD的反应率分别为14.0%、0.5%和8.5%，对PPD的反应率分别为6%、0%和6%，对POX的反应率分别为7.5%、0%和1%。分离的细胞和未分离的细胞对抗原的反应，而不是对PHA的反应，取决于培养中细胞的密度。Leighton管的最适细胞浓度为每次培养3~4×106个淋巴细胞和0.2~0.6×106个巨噬细胞。用半透膜分离淋巴细胞和巨噬细胞，阻止了原始细胞的恢复。这些结果与巨噬细胞摄取抗原和巨噬细胞-淋巴细胞相互作用是淋巴细胞体外对抗原产生胚性反应所必需的观点一致。
Glass bead column purified lymphocyte suspensions have reduced blastogenic responses to antigen when compared to unseparated leukocyte suspensions in vitro. Culture of these purified lymphocytes on macrophage monolayers generally restored the cells response to antigen. The response to phytohemagglutinin (PHA) was unaffected by column purification. The antigens used were streptolysin O (SLO), streptokinase-streptodornase (SK-SD), purified protein derivative (PPD) and small pox vaccine (POX). The response of the unseparated leukocytes, separated lymphocytes and separated lymphocytes on monolayers were 76%, 74% and 74% blasts generated in response to PHA; 18%, 0.0% and 10.0% blasts generated in response to SLO; 14.0%, 0.5% and 8.5% in response to SK-SD; 6%, 0% and 6% in response to PPD; and 7.5%, 0% and 1% in response to POX. The response of separated and unseparated cells to antigen but not to PHA was found to be dependent on the density of cells in culture. For Leighton tubes the optimal cell concentrations were 3 to 4 × 106 lymphocytes and 0.2 to 0.6 × 106 macrophages per culture. Separation of the lymphocytes and macrophages by a semi-permeable membrane prevented the restoration of blastogenesis. These results are consistent with the view that macrophage uptake of antigen and macrophage-lymphocyte interaction are necessary for the blastogenic response of lymphocytes to antigen in vitro.