Dickkopf genes are co-ordinately expressed in mesodermal lineages
Dickkopf genes are co-ordinately expressed in mesodermal lineages
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DOI：
10.1016/s0925-4773(99)00138-0
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发表时间：
1999-09-01
影响因子：
2.6
通讯作者：
Niehrs, C
中科院分区：
文献类型：
--
作者：
Monaghan, AP;Kioschis, P;Niehrs, C
Dickkopf-1（dkk-1）是一个新的分泌蛋白家族的成员，在非洲爪蟾胚胎发育过程中的头部诱导中起作用，作为Wnt信号传导的有效抑制剂。我们在此报告：（1）分离dkk家族的另外两个鼠成员dkk-2和dkk-3;和（2）分析小鼠dkk-1、dkk-2和dkk-3、非洲爪蟾dkk-1以及鸡dkk-3的成体和胚胎基因表达。对小鼠dkk-1、dkk-2和dkk-3基因的比较发育分析表明，这些基因在时间和空间上都受到调控。它们定义了间充质谱系中的重叠深层结构域，表明了协调的作用模式。所有dkks在介导上皮-间充质转化的组织中显示出不同的和升高的表达模式，这表明它们可能参与心脏、牙齿、毛发和胡须毛囊、肢体和骨诱导。在肢芽中，这些基因的表达存在于程序性细胞死亡的区域。在给定的器官中，dkk-1往往是最早表达的成员。与非洲爪蟾dkk-1和鸡dkk-3的比较显示进化上保守的表达模式。我们的观察表明，dkk基因构成了一个新的分泌蛋白家族，可能介导上皮细胞和间充质细胞之间的诱导相互作用。(C)1999爱思唯尔科学爱尔兰有限公司保留所有权利。
Dickkopf-1 (dkk-1) is member of a novel family of secreted proteins and functions in head induction during Xenopus embryogenesis, acting as a potent inhibitor of Wnt signalling. Here we report: (1) the isolation of two additional murine members of the dkk family, dkk-2 and dkk-3; and (2) analysis of adult and embryonic gene expression of mouse dkk-1,-2, and -3, Xenopus dkk-1 as well as chicken dkk-3. Comparative developmental analyses of the dkk-1, dkk-2 and dkk-3 in mice indicate that these genes are both temporally and spatially regulated. They define overlapping deep domains in mesenchymal lineages suggesting a co-ordinated mode of action. All dkks show distinct and elevated expression patterns in tissues that mediate epithelial-mesenchyme transformations suggesting that they may participate in heart, tooth, hair and whisker follicle, limb and bone induction. In the limb buds expression of these genes are found in regions of programmed cell death. In a given organ, dkk-1 tends to be the earliest member expressed. Comparison with Xenopus dkk-1 and chicken dkk-3 shows evolutionarily conserved expression patterns. Our observations indicate that dkk genes constitute a new family of secreted proteins that may mediate inductive interactions between epithelial and mesenchymal cells. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.